Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/31168
Title: Peptides from Paracoccidioides brasiliensis GP43 inhibit macrophage functions and inflammatory response
Authors: Konno, Adriana Y. C. [UNIFESP]
Maricato, Juliana Terzi [UNIFESP]
Konno, Fabiana T. C. [UNIFESP]
Mariano, Mario [UNIFESP]
Lopes, Jose Daniel [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Keywords: Peptides
Paracoccidioides brasiliensis
GP43
Macrophage
Inflammation
Issue Date: 1-Jan-2009
Publisher: Elsevier B.V.
Citation: Microbes and Infection. Amsterdam: Elsevier B.V., v. 11, n. 1, p. 92-99, 2009.
Abstract: Paracoccidioidomycosis (PCM) is a systemic granulomatous disease caused by Paracoccidioides brasiliensis (Pb), a thermal dimorphic fungus. Its major antigen is a 43-kDa glycoprotein. Gp43 embodies different functions: it participates in evasion mechanisms during the installation of primary infection, stimulates granuloma-like formation in vitro and presents T-cell epitopes that induce protective response against the fungus. Here, we investigated epitopes from gp43 inhibitory of both, macrophage functions and inflammatory reaction. Different gp43 peptides, spanning the entire sequence of the molecule, were added to cultures of bone marrow-derived macrophages. After challenge with zymosan or Pb cells, phagocytic indexes were measured. Peptides expressed on the molecule surface were determined by graphic analysis using the Protean module; DNAstar Inc. Two peptides which decreased phagocytic index and were expressed at the surface of the molecule, P4 and P23, were selected for further studies. It was shown that both inhibited the release of NO by zymosan stimulated macrophages while enhanced release of H2O2. the release of TNF-alpha in culture supernatants from in vitro phagocytic tests showed different response depending of P4 concentration (data not shown). in vivo assays with Mycobacterium bovis - bacillus Calmette-Guerin (BCG) or Pb cells demonstrated that these peptides presented non-specific and specific anti-inflammatory properties. (c) 2008 Elsevier Masson SAS. All rights reserved.
URI: http://repositorio.unifesp.br/handle/11600/31168
ISSN: 1286-4579
Other Identifiers: http://dx.doi.org/10.1016/j.micinf.2008.10.011
Appears in Collections:Em verificação - Geral

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.