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Title: Hyperbaric oxygen therapy protects the liver from apoptosis caused by ischemia-reperfusion injury in rats
Authors: Chaves, Jose C.
Fagundes, Djalma José [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Bertoletto, Paulo Roberto
Oshima, Celina Tizuko Fujiyama [UNIFESP]
Taha, Murched Omar [UNIFESP]
Simões, Ricardo Santos [UNIFESP]
Fagundes, Anna Luiza Negrini
Fed Univ Grande Dourados
Universidade Federal de São Paulo (UNIFESP)
Univ Santo Amaro
Issue Date: 1-Jan-2009
Publisher: Wiley-Blackwell
Citation: Microsurgery. Hoboken: Wiley-liss, v. 29, n. 7, p. 578-583, 2009.
Abstract: Purpose: the present paper aimed to investigate the role of hyperbaric oxygen treatment (HBO) and the apoptosis in rat liver ischemia-reperfusion injury (IRI). Methods: Thirty-seven male Wistar rats were subjected to 30 minutes of hepatic ischemia and 30 minutes of reperfusion and randomly distributed into six groups: G-I/R (n = 8), control without HBO; G-HBO/I (n = 8), HBO only during the ischemia period; G-HBO/R (n = 8), HBO only during the reperfusion period; G-HBO-I/R (n = 8), HBO during both the ischemia and reperfusion periods; G-Sh (n = 3), HBO without ischemia or reperfusion as sham group; G-C (n = 2) for control of current apoptosis expression on the normal liver tissue. HBO was carried out using a transparent, cylindrical acrylic chamber with a pressure of 2.0 ATA. Hepatic samples were stained for caspase-3 cleavage. Results: Apoptotic cells were identified in all groups. in the hepatic specimens of animals HBO-treated during ischemia (GHBO-I), there was a significant decrease (P < 0.001) in the number of cells undergoing apoptosis (1.62 +/- 0.91). the apoptotic index showed no significant difference in the animals HBO-treated during ischemia/reperfusion (5.75 +/- 1.28) compared with the G-I/R (3.5 +/- 0.75), which had no HBO treatment. the apoptosis index (11.25 +/- 1.90) was significantly higher (P < 0.01) in HBO-treated animals during the reperfusion period when compared with any of the other groups. Conclusion: A favorable effect was obtained when hyperbaric oxygen was administered early during ischemia. the hyperbaric oxygen in later periods of reperfusion was associated with a more severe apoptosis index. (C) 2009 Wiley-Liss, Inc. Microsurgery 29:578-583, 2009.
ISSN: 0738-1085
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