Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/31032
Title: Dietary fish oil did not prevent sleep deprived rats from a reduction in adipose tissue adiponectin gene expression
Authors: Mattos, Ana Barbosa Marcondes de [UNIFESP]
Pinto, Monica Jordao S. [UNIFESP]
Oliveira, Cristiane [UNIFESP]
Biz, Carolina [UNIFESP]
Ribeiro, Eliane Beraldi [UNIFESP]
Nascimento, Claudia Maria da Penha Oller do [UNIFESP]
Andersen, Monica Levy [UNIFESP]
Tufik, Sergio [UNIFESP]
Oyama, Lila Missae [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Issue Date: 5-Nov-2008
Publisher: Biomed Central Ltd
Citation: Lipids in Health and Disease. London: Biomed Central Ltd, v. 7, 11 p., 2008.
Abstract: Sleep deprivation in humans has been related to weight gain and consequently, increased risk for insulin resistance. in contrast, there is a significant loss of weight in sleep deprived rats suggesting a state of insulin resistance without obesity interference. Thus, we aimed to assess the effects of a rich fish oil dietetic intervention on glucose tolerance, serum insulin and adiponectin, and adipose tissue gene expression of adiponectin and TNF-alpha of paradoxically sleep deprived (PSD) rats. the study was performed in thirty day-old male Wistar randomly assigned into two groups: rats fed with control diet ( soybean oil as source of fat) and rats fed with a fish oil rich diet. After 45 days of treatment, the animals were submitted to PSD or maintained as home cage control group for 96 h. Body weight and food intake were carefully monitored in all groups. At the end of PSD period, a glucose tolerance test was performed and the total blood and adipose tissues were collected. Serum insulin and adiponectin were analyzed. Adipose tissues were used for RT-PCR to estimate the gene expression of adiponectin and TNF-alpha. Results showed that although fish oil diet did not exert any effect upon these measurements, PSD induced a reduction in adiponectin gene expression of retroperitoneal adipose tissues, with no change in serum adiponectin concentration or in adiponectin and TNF-alpha gene expression of epididymal adipose tissue. Thus, the stress induced by sleep deprivation lead to a desbalance of adiponectin gene expression.
URI: http://repositorio.unifesp.br/handle/11600/31032
ISSN: 1476-511X
Other Identifiers: http://dx.doi.org/10.1186/1476-511X-7-43
Appears in Collections:Em verificação - Geral

Files in This Item:
File Description SizeFormat 
WOS000262253800001.pdf439.14 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.