Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/30757
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dc.contributor.authorScrideli, Carlos A.
dc.contributor.authorCarlotti, Carlos G.
dc.contributor.authorOkamoto, Oswaldo K. [UNIFESP]
dc.contributor.authorAndrade, Vanessa S.
dc.contributor.authorCortez, Maria A. A.
dc.contributor.authorMotta, Fabio J. N.
dc.contributor.authorLucio-Eterovic, Agda K.
dc.contributor.authorNeder, Luciano
dc.contributor.authorRosemberg, Sergio
dc.contributor.authorOba-Shinjo, Sueli M.
dc.contributor.authorMarie, Suely K. N.
dc.contributor.authorTone, Luiz G.
dc.date.accessioned2016-01-24T13:51:30Z-
dc.date.available2016-01-24T13:51:30Z-
dc.date.issued2008-07-01
dc.identifierhttp://dx.doi.org/10.1007/s11060-008-9579-4
dc.identifier.citationJournal of Neuro-oncology. New York: Springer, v. 88, n. 3, p. 281-291, 2008.
dc.identifier.issn0167-594X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30757-
dc.description.abstractThe prognosis of glioblastomas is still extremely poor and the discovery of novel molecular therapeutic targets can be important to optimize treatment strategies. Gene expression analyses comparing normal and neoplastic tissues have been used to identify genes associated with tumorigenesis and potential therapeutic targets. We have used this approach to identify differentially expressed genes between primary glioblastomas and non-neoplastic brain tissues. We selected 20 overexpressed genes related to cell cycle, cellular movement and growth, proliferation and cell-to-cell signaling and analyzed their expression levels by real time quantitative PCR in cDNA obtained from microdissected fresh tumor tissue from 20 patients with primary glioblastomas and from 10 samples of non-neoplastic white matter tissue. the gene expression levels were significantly higher in glioblastomas than in non-neoplastic white matter in 18 out of 20 genes analyzed: P < 0.00001 for CDKN2C, CKS2, EEF1A1, EMP3, PDPN, BNIP2, CA12, CD34, CDC42EP4, PPIE, SNAI2, GDF15 and MMP23b; and NFIA (P: 0.0001), GPS1 (P: 0.0003), LAMA1 (P: 0.002), STIM1 (P: 0.006), and TASP1 (P: 0.01). Five of these genes are located in contiguous loci at 1p31-36 and 2 at 17q24-25 and 8 of them encode surface membrane proteins. PDPN and CD34 protein expression were evaluated by immunohistochemistry and they showed concordance with the PCR results. the present results indicate the presence of 18 overexpressed genes in human primary glioblastomas that may play a significant role in the pathogenesis of these tumors and that deserve further functional investigation as attractive candidates for new therapeutic targets.en
dc.format.extent281-291
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofJournal of Neuro-oncology
dc.rightsAcesso restrito
dc.subjectglioblastomaen
dc.subjectbrainen
dc.subjectgene expressionen
dc.subjectmicroarrayen
dc.subjectRQ-PCRen
dc.titleGene expression profile analysis of primary glioblastomas and non-neoplastic brain tissue: identification of potential target genes by oligonucleotide microarray and real-time quantitative PCRen
dc.typeArtigo
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniv São Paulo, Dept Puericultura & Pediat, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Pediat, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Surg, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, BR-04023900 São Paulo, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Genet, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, Brazil
dc.description.affiliationUniv São Paulo, Dept Pathol, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Pathol, Fac Med, BR-01246903 São Paulo, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Neurol, Fac Med, BR-01246903 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, BR-04023900 São Paulo, SP, Brazil
dc.identifier.doi10.1007/s11060-008-9579-4
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000256339900005
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