Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/30717
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dc.contributor.authorAtayde, Vanessa [UNIFESP]
dc.contributor.authorJasiulionis, Miriam G. [UNIFESP]
dc.contributor.authorCortez, Mauro [UNIFESP]
dc.contributor.authorYoshida, Nobuko [UNIFESP]
dc.date.accessioned2016-01-24T13:51:27Z
dc.date.available2016-01-24T13:51:27Z
dc.date.issued2008-06-01
dc.identifierhttp://dx.doi.org/10.1097/CMR.0b013e3282feeaab
dc.identifier.citationMelanoma Research. Philadelphia: Lippincott Williams & Wilkins, v. 18, n. 3, p. 172-183, 2008.
dc.identifier.issn0960-8931
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30717
dc.description.abstractTrypanosoma cruzi infection is known to confer resistance to tumor development in mice, and in-vitro studies have shown the toxic effects of parasite extracts on cancer cell cultures. Investigations in which T cruzi molecules exhibit antitumor activity have just begun. Here, we used a tumorigenic cell line Tm5, derived from mouse melanocytes melan-a, to test the effect of J18, a recombinant protein based on T cruzi surface molecule gp82 fused to glutathione-S-transferase (GST). J18 induced actin cytoskeleton disruption in Tm5 but not in melan-a cells. Several changes indicative of apoptosis were detected in Tm5 melanoma cells but not in melan-a cells treated with J18, such as the flipping of phosphatidylserine from the inner to the external side of the plasma membrane, altered nuclear morphology, DNA fragmentation, increase in mitochondria depolarization, and in caspase-3 activity. Retention of NF-kappa B in the cytoplasm was another alteration observed specifically in J18-treated Tm5 cells. No such alterations were found in Tm5 cells treated with GST. In-vivo experiments showed that C57BL/6 mice inoculated with Tm5 cells, treated at the site of tumor cell inoculation with J18, developed tumors of smaller size than mice treated with phosphate-buffered saline or GST and survived longer.en
dc.format.extent172-183
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofMelanoma Research
dc.rightsAcesso restrito
dc.subjectsurface molecule gp82en
dc.subjectmelanoma cellsen
dc.subjectTrypanosoma cruzien
dc.titleA recombinant protein based on Trypanosoma cruzi surface molecule gp82 induces apoptotic cell death in melanoma cellsen
dc.typeArtigo
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pharmacol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pharmacol, BR-04023062 São Paulo, Brazil
dc.identifier.doi10.1097/CMR.0b013e3282feeaab
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000256332300003
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