Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/30693
Title: Occurrence of antibodies to Paracoccidioides brasiliensis in dairy cattle from Mato Grosso do Sul, Brazil
Authors: Silveira, L. H.
Paes, R. C. S.
Medeiros, E. V.
Itano, E. N.
Camargo, Z. P. [UNIFESP]
Ono, M. A.
Universidade Estadual de Londrina (UEL)
Fac Luiz Meneghel
IAGRO
Universidade Federal de São Paulo (UNIFESP)
Keywords: paracoccidioidomycosis
bovine
epidemiology
ELISA
Issue Date: 1-Jun-2008
Publisher: Springer
Citation: Mycopathologia. Dordrecht: Springer, v. 165, n. 6, p. 367-371, 2008.
Abstract: The aim of this study was to evaluate the humoral immune response in cattle immunized with Paracoccidioides brasiliensis and perform a seroepidemiological study of paracoccidioidomycosis in dairy cattle from Mato Grosso do Sul. Two animals (one steer and one heifer) were inoculated with a suspension of P. brasiliensis in Freund incomplete adjuvant. Blood samples were collected periodically to evaluate humoral immune response by immunodiffusion and ELISA, using exoantigen and gp43 as antigens, respectively. the antibody production was detected by immunodiffusion and ELISA, in both animals, 14 days after immunization. the soroepidemiologic study was carried out in 400 cattle of Mato Grosso do Sul from four municipalities: Corumba, Dourados, Nova Andradina, and Sao Gabriel d'Oeste. the municipalities of Corumba (30%) and Nova Andradina (28%) showed higher positivity than Dourados (8%) and Sao Gabriel d'Oeste (4%). in this study we concluded that cattle immunized with P. brasiliensis develop humoral immune response for gp43, remaining with high titers of antibodies, and that this animal species could be an epidemiologic marker of paracoccidioidomycosis.
URI: http://repositorio.unifesp.br/handle/11600/30693
ISSN: 0301-486X
Other Identifiers: http://dx.doi.org/10.1007/s11046-008-9095-2
Appears in Collections:Em verificação - Geral

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