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Title: Interaction of epithelial cell membrane rafts with Paracoccidioides brasiliensis leads to fungal adhesion and Src-family kinase activation
Authors: Maza, Paloma K. [UNIFESP]
Straus, Anita H. [UNIFESP]
Toledo, Marcos S. [UNIFESP]
Takahashi, Helio K. [UNIFESP]
Suzuki, Erika [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Keywords: membrane microdomains
Paracoccidioides brasiliensis
host-pathogen interactions
Src-family kinases
ERK MAP kinases
Issue Date: 1-Apr-2008
Publisher: Elsevier B.V.
Citation: Microbes and Infection. Amsterdam: Elsevier B.V., v. 10, n. 5, p. 540-547, 2008.
Abstract: Membrane rafts are cholesterol- and sphingolipid-enriched cell membrane domains, which are ubiquitous in mammals and play an essential role in different cellular functions, including host cell-pathogen interaction. in this work, by using several approaches, we demonstrated the involvement of epithelial cell membrane rafts in adhesion process of the pathogenic fungus Paracoccidioides brasiliensis. This conclusion was supported by the localization of ganglioside GM1, a membrane raft marker, at P. brasiliensis-epithelial cell contact sites, and by the inhibition of this fungus adhesion to host cells pre-treated with cholesterol-extractor (methyl-beta-cyclodextrin, M beta CD) or -binding (nystatin) agents. in addition, at a very early stage of P. brasiliensis-A549 cell interaction, this fungus promoted activation of Src-family kinases (SFKs) and extracellular signal-regulated kinase 1/2 (ERK1/2) of these epithelial cells. Whereas SFKs were partially responsible for activation of ERK1/2, membrane raft disruption with M beta CD in A549 cells led to total inhibition of SFK activation. Taking together, these data indicate for the first time that epithelial cell membrane rafts are essential for P. brasiliensis adhesion and activation of cell signaling molecules. (C) 2008 Elsevier Masson SAS. All rights reserved.
ISSN: 1286-4579
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