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Title: Genomic gains of COLIAI-PDFGB occur in the histologic evolution of giant cell fibroblastoma into dermatofibrosarcoma protuberans
Authors: Macarenco, Ricardo S.
Zamolyi, Renata
Franco, Marcello F. [UNIFESP]
Nascimento, Antonio G.
Abott, Jared J.
Wang, Xiaoke
Erickson-Johnson, Michele R.
Oliveira, Andre M.
Mayo Clin
Universidade Federal de São Paulo (UNIFESP)
Issue Date: 1-Mar-2008
Publisher: Wiley-Blackwell
Citation: Genes Chromosomes & Cancer. Hoboken: Wiley-liss, v. 47, n. 3, p. 260-265, 2008.
Abstract: Giant cell fibroblastoma (GCF) is a subcutaneous mesenchymal neoplasm characterized by the chromosomal t(17;22), which results in the formation of the fusion gene COLIAI-PDGFB. This same fusion gene is also seen in the supernumerary ring chromosome of dermatofibrosarcoma protuberans (DFSP). Several studies have addressed the molecular genetics of DFSP but molecular cytogenetic characterization of individual areas and cell components in pure GCF and GCF/DFSP hybrids have not been performed. Herein, we studied the frequency and genomic copy number of COLIAI-PDGFB in pure GCF and GCF/DFSP hybrids, and identified the molecular cytogenetic signatures in individual cells in each component. Four pure GCF and nine GCF/DFSP hybrids were studied. All tumors exhibited classical histological features and CD34 expression. COLIAI and PDGFB rearrangements were evaluated by fluorescence in situ hybridization (FISH) using probes for COLIAI and PDGFB on paraffin-embedded thin tissue sections. All GCF and GCF/DFSP hybrids showed unbalanced rearrangements of COLIAI-PDGFB at the molecular cytogenetic level. Genomic gains of COLIAI-PDGFB were found predominantly in the DFSP component of GCF/DFSP hybrids but in none of the pure GCF, suggesting that these gains are associated with the histologic evolution of GCF into DFSP. the molecular cytogenetic abnormalities were found not only in the spindle/stellated cells but also in individual nuclei of the multinucleated giant cells, suggesting that these cells may result from the fusion of individual neoplastic cells. (c) 2007 Wiley-Liss, Inc.
ISSN: 1045-2257
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