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Title: Cathepsin V, but not cathepsins L, B and K, may release angiostatin-like fragments from plasminogen
Authors: Puzer, Luciano
Barros, Nilana M. T. [UNIFESP]
Paschoalin, Thaysa [UNIFESP]
Hirata, Izaura Y. [UNIFESP]
Tanaka, Aparecida S. [UNIFESP]
Oliveira, Marcelo C.
Broemme, Dieter
Carmona, Adriana K. [UNIFESP]
Univ British Columbia
Universidade Federal de São Carlos (UFSCar)
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Keywords: angiogenesis
corneal epithelium
cysteine cathepsins
Issue Date: 1-Feb-2008
Publisher: Walter de Gruyter & Co
Citation: Biological Chemistry. Berlin: Walter de Gruyter & Co, v. 389, n. 2, p. 195-200, 2008.
Abstract: Cathepsin V is a lysosomal cysteine peptidase highly expressed in corneal epithelium; however, its function in the eye is still unknown. Here, we describe the capability of cathepsin V to hydrolyze plasminogen, which is also expressed in human cornea at levels high enough to produce physiologically relevant amounts of angiostatin-related molecules. the co-localization of these two proteins suggests an important role for the enzyme in the maintenance of corneal avascularity, essential for optimal visual performance. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of plasminogen digestion by cathepsin V revealed the generation of three major products of 60, 50 and 40 kDa, which were electrotransferred to polyvinylidene difluoride membranes and excised for characterization. NH2-terminal amino acid sequencing of these fragments revealed the sequences EKKVYL, TEQLAP and LLPNVE, respectively. These data are compatible with cleavage sites at plasminogen F94-E95, S358-T359 and V468-L469 peptide bonds generating fragments of the five-kringle domains. in contrast, we did not detect any plasminogen degradation by cathepsins B, K and L. Using a Matrigel assay, we confirmed the angiogenesis inhibition activity on endothelial cells caused by plasminogen processing by cathepsin V. Our results suggest a novel physiological role for cathepsin V related to the control of neovascularization in cornea.
ISSN: 1431-6730
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