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Title: Expression of angiotensin I-converting enzymes and bradykinin B-2 receptors in mouse inner medullary-collecting duct cells
Authors: Redublo Quinto, Beata Marie [UNIFESP]
Camargo de Andrade, Maria Claudina [UNIFESP]
Ronchi, Fernanda Aparecida [UNIFESP]
Santos, Edson Lucas
Corrêa, Silvana Aparecida Alves [UNIFESP]
Shimuta, Suma Imura
Pesquero, Joao Bosco
Mortara, Renato Arruda
Casarini, Dulce Elena [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Dept Biofis
Dept Microbiol Imunol & Parasitol
Keywords: angiotensin I-converting enzyme
mIMCD-3 cells
kinin receptors
Issue Date: 1-Feb-2008
Publisher: Elsevier B.V.
Citation: International Immunopharmacology. Amsterdam: Elsevier B.V., v. 8, n. 2, p. 254-260, 2008.
Abstract: We described in mouse inner medullary-collecting duct cells (m1MCD-3) the somatic and the N-domain ACE synthesis and its interaction with the kallikrein-kinin system co-localized in the same cells. We purified two ACE forms from culture medium, M1 (130 kDa) and M2 (N-domain, 60 kDa), and cellular lysate, C1 (130 kDa) and C2 (N-domain, 60 kDa). Captopril. and enalaprilat inhibited the purified enzymes. the immunofluorescence studies indicated that ACE is present in the membrane, cytoplasm and in the cell, nucleus. Kinin B-1 and B-2 receptors were detected by immunofluorescence and showed to be activated by BK and DesR(9) BK, increasing the acidification rate which was enhanced in the presence of enalaprilat. the presence of secreted and intracellular ACE in mIMCD-3 confirmed the hypothesis previously proposed by our group for a new site of ACE secretion in the collecting duct. (c) 2007 Elsevier B.V. All rights reserved.
ISSN: 1567-5769
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