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Title: Usefulness of a quick decalcification of bone sections embedded in methyl metacrylate: an improved method for immunohistochemistry
Authors: Gomes, Samirah Abreu [UNIFESP]
Reis, Luciene Machado dos
Oliveira, Ivone Braga de
Noronha, Irene de Lourdes
Jorgetti, Vanda
Heilberg, Ita Pfeferman [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Keywords: bone
methyl methacrylate
chronic kidney disease
Issue Date: 1-Jan-2008
Publisher: Springer
Citation: Journal of Bone and Mineral Metabolism. Tokyo: Springer Tokyo, v. 26, n. 1, p. 110-113, 2008.
Abstract: Immunohistochemistry of undecalcified bone sections embedded in methyl methacrylate (MMA) is not commonly employed because of potential destruction of tissue antigenicity by highly exothermic polymerization. the aim of the present study was to describe a new technique in which a quick decalcification of bone sections embedded in MMA improves the results for immunohistochemistry. the quality of interleukin 1 alpha (IL-1 alpha) immunostaining according to the present method was better than the conventional one. Immunostaining for osteoprotegerin (OPG) and the receptor activator of NF-kappa B ligand (RANKL) in bone sections of chronic kidney disease patients with mineral bone disorders (CKD-MBD) was stronger than in controls (postmortem healthy subjects). the present study suggested that this method is easy, fast, and effective to perform both histomorphometry and immunohistochemistry in the same bone fragment, yielding new insights into pathophysiological aspects and therapeutic approaches in bone disease.
ISSN: 0914-8779
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