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Title: Metacaspase 2 of Trypanosoma brucei is a calcium-dependent cysteine peptidase active without processing
Authors: Moss, Catherine X.
Westrop, Gareth D.
Juliano, Luiz [UNIFESP]
Coombs, Graham H.
Mottram, Jeremy C.
Univ Glasgow
Univ Strathclyde
Universidade Federal de São Paulo (UNIFESP)
Keywords: caspase
cysteine protease
Issue Date: 11-Dec-2007
Publisher: Elsevier B.V.
Citation: Febs Letters. Amsterdam: Elsevier B.V., v. 581, n. 29, p. 5635-5639, 2007.
Abstract: Metacaspases are cysteine peptidases that are distantly related to the caspases, for which proteolytic processing is central to their activation. Here, we show that recombinant metacaspase 2 (MCA2) from Trypanosoma brucei has arginine/lysine-specific, Ca2+-dependent proteolytic activity. Autocatalytic processing of MCA2 occurred after Lys55 and Lys268; however, this was shown not to be required for the enzyme to be proteolytically active. the necessity of Ca2+, but not processing, for MCA2 enzymatic activity clearly distinguishes MCA2 from the caspases and would be consistent with different physiological roles. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
ISSN: 0014-5793
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