Please use this identifier to cite or link to this item:
https://repositorio.unifesp.br/handle/11600/29845| Title: | Pulsed-field gel electrophoresis in the identification of the origin of bacterial keratitis caused by Pseudomonas aeruginosa |
| Authors: | Melo, Gustavo Barreto de Aggio, Fabio Bom Hofling-Lima, Ana Luisa d'Azevedo, Pedro Alves [UNIFESP] Campos Pignatari, Antonio Carlos Universidade Federal de São Paulo (UNIFESP) |
| Keywords: | pulsed-field gel electrophoresis bacterial keratitis Pseudomonas aeruginosa |
| Issue Date: | 1-Jul-2007 |
| Publisher: | Springer |
| Citation: | Graefes Archive for Clinical and Experimental Ophthalmology. New York: Springer, v. 245, n. 7, p. 1053-1054, 2007. |
| Abstract: | Purpose To report the use of pulsed-field gel electrophoresis (PFGE) in the investigation of the origin of infectious keratitis.Methods A 19-year-old girl presented with infectious keratitis in the left eye. She was a soft contact lens wearer, and was noncompliant with the proper cleaning methods proposed by the manufacturer. Microbiological assessment was performed by means of culture and PFGE.Results the patient was treated successfully with topical cefalotin and gentamicin drops. Cultures were positive for Pseudomonas aeruginosa in the cornea and conjunctiva, and in the contact lens, its case and the cleaning solution. PFGE showed identical patterns of banding in each.Conclusion in this case of bacterial keratitis, PFGE proved to be very useful in identifying how the contamination occurred. |
| URI: | http://repositorio.unifesp.br/handle/11600/29845 |
| ISSN: | 0721-832X |
| Other Identifiers: | http://dx.doi.org/10.1007/s00417-006-0503-3 |
| Appears in Collections: | Em verificação - Geral |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.