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|Title:||Inhibitory input from slowly adapting lung stretch receptors to retrotrapezoid nucleus chemoreceptors|
|Authors:||Moreira, Thiago S. [UNIFESP]|
Takakura, Ana C. [UNIFESP]
Colombari, Eduardo [UNIFESP]
West, Gavin H.
Guyenet, Patrice G.
Universidade Federal de São Paulo (UNIFESP)
|Citation:||Journal of Physiology-london. Oxford: Blackwell Publishing, v. 580, n. 1, p. 285-300, 2007.|
|Abstract:||The retrotrapezoid nucleus (RTN) contains CO2-activated interneurons with properties consistent with central respiratory chemoreceptors. These neurons are glutamatergic and express the transcription factor Phox2b. Here we tested whether RTN neurons receive an input from slowly adapting pulmonary stretch receptors (SARs) in halothane-anaesthetized ventilated rats. in vagotomized rats, RTN neurons were inhibited to a variable extent by stimulating myelinated vagal afferents using the lowest intensity needed to inhibit the phrenic nerve discharge (PND). in rats with intact vagus nerves, RTN neurons were inhibited, also to a variable extent, by increasing positive end-expiratory pressure (PEEP; 2-6 cmH(2)O). the cells most sensitive to PEEP were inhibited during each lung inflation at rest and were instantly activated by stopping ventilation. Muscimol (GABA-A agonist) injection in or next to the solitary tract at area postrema level desynchronized PND from ventilation, eliminated the lung inflation-synchronous inhibition of RTN neurons and their steady inhibition by PEEP but did not change their CO2 sensitivity. Muscimol injection into the rostral ventral respiratory group eliminated PND but did not change RTN neuron response to either lung inflation, PEEP increases, vagal stimulation or CO2. Generalized glutamate receptor blockade with intracerebroventricular (I.C.V) kynurenate eliminated PND and the response of RTN neurons to lung inflation but did not change their CO2 sensitivity. PEEP-sensitive RTN neurons expressed Phox2b. in conclusion, RTN chemoreceptors receive an inhibitory input from myelinated lung stretch receptors, presumably SARs. the lung input to RTN may be di-synaptic with inhibitory pump cells as sole interneurons.|
|Appears in Collections:||Em verificação - Geral|
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