Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/29469
Title: Cloning, characterization and expression of a calnexin homologue from the pathogenic fungus Paracoccidioides brasiliensis
Authors: Feitosa, Luciano dos Santos [UNIFESP]
Maria de Almeida Soares, Celia
Santos, Marcia Regina Machado [UNIFESP]
Melo Bailao, Alexandre
Xander, Patricia [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
Daniel Lopes, Jose [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Goiás (UFG)
Keywords: chaperone
calnexin homologue
recombinant protein
paracoccidioidomycosis
Paracoccidioidides brasiliensis
Issue Date: 1-Feb-2007
Publisher: Wiley-Blackwell
Citation: Yeast. Chichester: John Wiley & Sons Ltd, v. 24, n. 2, p. 79-87, 2007.
Abstract: We report the cloning of a Paracoccidioides brasiliensis cDNA, here named PbCnx, encoding the homologue of the endoplasmic reticulum molecular chaperone calnexin. Calnexin specifically recognizes monoglucosylated glycoproteins in the endoplasmic reticulum, thus being an essential component of the complex that interacts with the folded state of nascent secreted glycoproteins. the PbCnx open reading frame was found in a 1701 base pair (bp) fragment that encodes a 567 amino acid protein with an estimated mass of 62 680 Da. Northern and Southern blot hybridizations showed that PbCnx is encoded by a single, or a low number of, gene copies. PbCnx contains the hallmark KPEDWD motifs that are found in all members of the calnexin/calreticulin family proteins. A cDNA-encoding PbCnx was overexpressed as recombinant protein in Escherichia coli. the purified recombinant PbCnx was recognized by 6 out of 10 sera from PCM patients, a result that rules out its possible consideration for further use in diagnosis. Using confocal microscopy with anti-PbCnx mouse serum against yeast forms, a cytoplasmic staining pattern was observed. Copyright (c) 2006 John Wiley & Sons, Ltd.
URI: http://repositorio.unifesp.br/handle/11600/29469
ISSN: 0749-503X
Other Identifiers: http://dx.doi.org/10.1002/yea.1438
Appears in Collections:Em verificação - Geral

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