Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/29468
Title: Virulence markers and genetic relationships of Shiga toxin-producing Escherichia coli strains from serogroup O111 isolated from cattle
Authors: Tristao, Luciana C. S.
Gonzalez, Alice G. M.
Coutinho, Cesar A. S.
Cerqueira, Aloysio M. F.
Gomes, Marcos J. P.
Irino, Kinue
Guth, Beatriz E. C.
Andrade, Joao R. C.
Universidade do Estado do Rio de Janeiro (UERJ)
Universidade Federal Fluminense (UFF)
Univ Fed Rio Grande Sul
Inst Adolfo Lutz Registro
Universidade Federal de São Paulo (UNIFESP)
Keywords: STEC
serogroup O111
virulence traits
healthy cattle
human diarrhea
Brazil
Issue Date: 31-Jan-2007
Publisher: Elsevier B.V.
Citation: Veterinary Microbiology. Amsterdam: Elsevier B.V., v. 119, n. 2-4, p. 358-365, 2007.
Abstract: Shiga toxin-producing Escherichia coli (STEC) strains isolated from healthy cattle (O111:NM, seven strains; O111:H8, three strains) in Brazil were studied and compared to previously characterized human strains in regard to their phenotypic and genotypic characteristics to evaluate their pathogenic potential. Most bovine STEC O111 strains were isolated from dairy calves, and strains with genotypes stx1 alone and stx1/stx2 (variant stx2) occurred in different regions. Irrespective of the stx genotype, all strains were positive for eae theta, alpha variants of tir, espA and espB, and for ler, qseA, iha, astA and efa1 genes. Only one strain was negative for EHEC-hlyA and all strains were negative for iha, saa and esPP genes and for EAF and bfpA, genetic markers of EPEC. Except for the presence of stx2, bovine strains showed the same profile of putative virulence genes found among the human strains. Similar biochemical behavior was identified among the strains analysed. Two bovine STEC strains produced the localized adherence (LA) phenotype in 6-h tests with Caco-2 (human enterocyte) cells. Intimate attachment (judged by the FAS test) was found in 9 out of 10 bovine strains as it was observed for the human STEC strains. RAPD-PCR analysis showed two distinct RAPD groups among the STEC O111 strains examined. Despite the relative low frequency of STEC O111 strains recovered from cattle no differences in their pathogenic potential were observed compared to some strains isolated from human diarrhea, suggesting that healthy cattle may be a potential source of infection for humans in Brazil. (c) 2006 Elsevier B.V. All rights reserved.
URI: http://repositorio.unifesp.br/handle/11600/29468
ISSN: 0378-1135
Other Identifiers: http://dx.doi.org/10.1016/j.vetmic.2006.09.006
Appears in Collections:Em verificação - Geral

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