Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/29314
Title: Structural and inhibitory properties of a plant proteinase inhibitor containing the RGD motif
Authors: Nakahata, Adriana M.
Bueno, Norlene R.
Rocha, Hugo A. O.
Franco, Celia R. C.
Chammas, Roger
Nakaie, Clovis R.
Jasiulionis, Miriam G.
Nader, Helena B.
Santana, Lucimeire A.
Sampaio, Misako U.
Oliva, Maria Luiza V.
Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande Norte
Univ Fed Parana
Universidade de São Paulo (USP)
Keywords: Bauhinia
elastase
cell adhesion
melanoma
RGD
trypsin inhibitor
tumor
Issue Date: 15-Dec-2006
Publisher: Elsevier B.V.
Citation: International Journal of Biological Macromolecules. Amsterdam: Elsevier B.V., v. 40, n. 1, p. 22-29, 2006.
Abstract: Purified from Bauhinia rufa seeds, BrTI is a Kunitz proteinase inhibitor that contains the RGD sequence. BrTI inhibits trypsin (K-iapp 2.9 nM) and human plasma kallikrein (K-iapp 14.0 nM) but not other related enzymes. the synthetic peptide YLEPVARGDGGLA-NH2 (70 mu M) inhibited the adhesion to fibronectin of B16F10 (high-metastatic B16 murine mouse melanoma cell line) and of Tm5 (murine melanoma cell lines derived from a non-tumorigenic lineage of pigmented murine melanocytes, melan-a). YLEPVARGEGGLA-NH2 in which Asp(9) was changed into Glu does not affect the cell attachment. Moreover, this peptide was functional only when the sequence present in the native protein was preserved, since YLIPVARGDGGLA-NH2 in which Glu(3) was changed into Ile does not interfere with B16F10 and was less effective on Tm5 cell line adhesion. Neither YLEPVARGDGGLA-NH2, YLIPVARGDGGLA-NH2 or YLEPVARGEGGLA-NH2 inhibit the interaction of RAEC (endothelial cell line from rabbit aorta) with fibronectin. (c) 2006 Elsevier B.V. All rights reserved.
URI: http://repositorio.unifesp.br/handle/11600/29314
ISSN: 0141-8130
Other Identifiers: http://dx.doi.org/10.1016/j.ijbiomac.2006.05.008
Appears in Collections:Em verificação - Geral

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.