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Title: An outbreak of keratitis caused by Mycobacterium immunogenum
Authors: Mello Sampaio, Jorge Luiz [UNIFESP]
Nassar Junior, Doraldo [UNIFESP]
Freitas, Denise de [UNIFESP]
Hoefling-Lima, Ana Luisa [UNIFESP]
Miyashiro, Kozue
Alberto, Fernando Lopes
Leao, Sylvia Cardoso [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Inst Fleury Ensino & Pesquisa
Issue Date: 1-Sep-2006
Publisher: Amer Soc Microbiology
Citation: Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 44, n. 9, p. 3201-3207, 2006.
Abstract: From 8 October to 12 November 2003, 36 patients underwent surgical correction of myopia in a São Paulo, Brazil, clinic. Five patients had clinical signs of infectious keratitis, and a Mycobacterium species with previously unreported patterns determined by PCR restriction enzyme analysis of the hsp65 gene and PCR restriction enzyme analysis of the 16S-23S rRNA internal transcribed spacer (ITS) was isolated from corneal scrapings from four of these patients. Subsequent evaluation by phenotypic tests and partial sequencing of the hsp65, sodA, rpoB, and 16S rRNA genes and the ITS supported the species identification as a variant of Mycobacterium immunogenum. the source of infection was not determined. the outbreak was caused by a single clone, as evidenced by identical pulsed-field gel electrophoresis and enterobacterial repetitive intergenic consensus-PCR profiles. This is the first report of an outbreak where this species was isolated from infected tissues.
ISSN: 0095-1137
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