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|Title:||Cells positive for microtubule-associated protein 1B (MAP 1B) are present along rat and human efferent ductules and epididymis|
Silva, A. M. da
Porto, C. S.
Universidade Federal de São Paulo (UNIFESP)
Natl Autonomous Univ Mexico
Univ N Carolina
male reproductive tract
|Citation:||Cell and Tissue Research. New York: Springer, v. 325, n. 1, p. 125-133, 2006.|
|Abstract:||Microtubule-associated protein 1B (MAP 1B) is a neuronal cytoskeleton marker with predominant expression in the developing nervous system. the present study provides evidence for the expression of this cytoskeleton protein in non-neuronal and neuronal cells along rat and human efferent ductules and epididymis (initial segment, caput, and cauda). Reverse transcription/polymerase chain reaction and Western blot analysis were used to confirm the presence of MAP 1B (mRNA and protein) in rat tissues. Immunohistochemical studies revealed MAP-1B-positive staining in columnar ciliated cells present in efferent ductules and in narrow cells located in the initial segment, in both rat and human. MAP-1B-positive basal cells, located underneath the columnar cells, were only identified in the initial segment and caput epididymidis of the rat. Qualitative analysis of tissues from 40-day-old and 120-day-old rats indicated that the number of MAP-1B-positive ciliated, narrow, and basal cells per tubule increased with sexual maturation. These immunoreactive cells did not stain for dopamine beta-hydroxylase or acetylcholinesterase, indicating that they were not adrenergic or cholinergic in nature. Immunohistochemical studies also revealed the presence of MAP-1B-positive staining in interstitial nerve fibers in caput and cauda epididymidis from both rat and human. Thus, the expression of MAP 1B is not confined to a specific cell type in rat and human efferent ductules and epididymis. the functional significance of this cytoskeleton protein in tissues from the male reproductive tract requires further investigation.|
|Appears in Collections:||Em verificação - Geral|
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