Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/28710
Title: The PbMDJ1 gene belongs to a conserved MDJ1/LON locus in thermodimorphic pathogenic fungi and encodes a heat shock protein that localizes to both the mitochondria and cell wall of Paracoccidioides brasiliensis
Authors: Batista, Wagner L. [UNIFESP]
Matsuo, Alisson L. [UNIFESP]
Ganiko, Luciane [UNIFESP]
Barros, Tania F. [UNIFESP]
Veiga, Thiago R. [UNIFESP]
Freymuller, Edna [UNIFESP]
Puccia, Rosana [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Issue Date: 1-Feb-2006
Publisher: Amer Soc Microbiology
Citation: Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 5, n. 2, p. 379-390, 2006.
Abstract: J-domain (Dnaj) proteins, of the Hsp40 family, are essential cofactors of their cognate Hsp70 chaperones, besides acting as independent chaperones. in the present study, we have demonstrated the presence of Mdj1, a mitochondrial DnaJ member, not only in the mitochondria, where it is apparently sorted, but also in the cell wall of Paracoccidioides brasiliensis, a thermodimorphic pathogenic fungus. the molecule (PbMdj1) was localized to fungal yeast cells using both confocal and electron microscopy and also How cytometry. the antirecombinant PbMdj1 antibodies used in the reactions specifically recognized a single 55-kDa mitochondrial and cell wall (alkaline beta-mercaptoethanol extract) component, compatible with the predicted size of the protein devoid of its matrix peptide-targeting signal. Labeling was abundant throughout the cell wall and especially in the budding regions; however, anti-PbMdj1 did not affect fungal growth in the concentrations tested in vitro, possibly due to the poor access of the antibodies to their target in growing cells. Labeled mitochondria stood preferentially close to the plasma membrane, and gold particles were detected in the thin space between them, toward the cell surface. We show that Mdj1 and the mitochondrial proteinase Lon homologues are heat shock proteins in P. brasiliensis and that their gene organizations are conserved among thermodimorphic fungi and Aspergillus, where the genes are adjacent and have a common 5' region. This is the first time a DnaJ member has been observed on the cell surface, where its function is speculative.
URI: http://repositorio.unifesp.br/handle/11600/28710
ISSN: 1535-9778
Other Identifiers: http://dx.doi.org/10.1128/EC.5.2.379-390.2006
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