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Title: Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections
Authors: Sampaio, JLM [UNIFESP]
Chimara, Erica
Ferrazoli, Lucilaine
Telles, Maria Alice da Silva
Del Guercio, VMF
Jerico, ZVN
Miyashiro, Kozue [UNIFESP]
Fortaleza, CMCB
Padoveze, M. C.
Leao, S. C. [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Fleury Ctr Med Diagnost
Inst Adolfo Lutz Registro
Inst Vozza Med & Diagnose LTDA
Ctr Vigilancia Epidemiol Prof Alexandre Vranjac
Keywords: infections
molecular epidemiology
Mycobacterium fortuitum
plastic surgery
Issue Date: 1-Feb-2006
Publisher: Blackwell Publishing
Citation: Clinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 12, n. 2, p. 142-149, 2006.
Abstract: A cluster of cases of post-augmentation mammaplasty surgical site infections occurred between 2002 and 2004 in Campinas, in the southern region of Brazil. Rapidly growing mycobacteria were isolated from samples from 12 patients. Eleven isolates were identified as Mycobacterium fortuitum and one as Mycobacterium porcinum by PCR-restriction digestion of the hsp65 gene. These 12 isolates, plus six additional M. fortuitum isolates from non-related patients, were typed by pulsed-field gel electrophoresis (PFGE) and three PCR-based techniques: 16S-23S rRNA internal transcribed spacer (ITS) genotyping; randomly amplified polymorphic DNA (RAPD) PCR; and enterobacterial repetitive intergenic consensus (ERIC) PCR. Four novel M. fortuitum allelic variants were identified by restriction analysis of the ITS fragment. One major cluster, comprising six M. fortuitum isolates, and a second cluster of two isolates, were identified by the four methods. RAPD-PCR and ITS genotyping were less discriminative than ERIC-PCR. ERIC-PCR was comparable to PFGE as a valuable complementary tool for investigation of this type of outbreak.
ISSN: 1198-743X
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