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Title: In vivo and in vitro effect of killed Propionibacterium acnes and its purified soluble polysaccharide on mouse bone marrow stem cells and dendritic cell differentiation
Authors: Squaiella, C. C.
Ananias, R. Z.
Mussalem, J. S.
Braga, E. G.
Rodrigues, E. G.
Travassos, L. R.
Lopes, J. D.
Longo-Maugeri, L. M.
Universidade Federal de São Paulo (UNIFESP)
Keywords: dendritic cells
Proionibacterium acnes
stem cells
Issue Date: 1-Jan-2006
Publisher: Elsevier B.V.
Citation: Immunobiology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 211, n. 1-2, p. 105-116, 2006.
Abstract: Among the effects exerted by Propionibacterium acnes, a most relevant one is its capacity to modulate the Th1/Th2 cellular immune response. This effect depends on the induction and activation of antigen presenting cells, mainly dendritic cells (DCs), whose number is increased in the peripheral blood of animals treated with this bacterium. A soluble P. acnes polysaccharide (PS) extract also acts on DCs, modulating a Th1 immune response. These data led us to investigate the role of P. acnes and its soluble PS on murine bone marrow (BM) DCs. Bone marrow cells were analyzed by flow cytometry, showing an increase of stem cells and DCs in P. acnes- or PS-treated animals. Culturing in the presence of granulocyte monocyte colony stimulating factor (GM-CSF) increased the in vitro differentiation and maturation of these cells into BM-derived DCs (CD11c(+) and MHC class II+). Maturation of DCs was determined by increased CD80 and CD86 expression, IL-4 and IL-12 production, reduction in phagocytic capacity and increase in the antigen presenting ability to primed or naive T lymphocytes. These data indicate that P. acnes as well as its PS can modulate BM stem cells, originating mature DCs, which are important mainly at the initial antigen contact. (c) 2005 Elsevier GmbH. All rights reserved.
ISSN: 0171-2985
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