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Title: A new Paracoccidioides brasiliensis 70-kDa heat shock protein reacts with sera from paracoccidioidomycosis patients
Authors: Bisio, L. C.
Silva, S. P.
Pereira, I. S.
Xavier, MAS
Venancio, E. J.
Puccia, R.
Soares, CMA
Felipe, MSS
Universidade de Brasília (UnB)
Universidade Federal de Goiás (UFG)
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Londrina (UEL)
Novo Nordisk Prod Farmaceut Brasil
Keywords: antigenic peptides
Paracoccidioides brasiliensis
protein expression
Issue Date: 1-Sep-2005
Publisher: Taylor & Francis Ltd
Citation: Medical Mycology. Abingdon: Taylor & Francis Ltd, v. 43, n. 6, p. 495-503, 2005.
Abstract: A cDNA coding for a new member of the 70-kDa heat shock proteins (HSP70) family from the dimorphic and pathogenic fungus, Paracoccidioides brasiliensis, was cloned and characterized. the cDNA-deduced sequence coded for 655 amino acid residues and showed 95% identity to a previously described P. brasiliensis hsp70 gene. Cytoplasmic and typical nuclear localization signals, which indicate induction upon stress, were identified in the deduced peptide. the complete hsp70 cDNA coding region was cloned into a pGEX 4T-3 plasmid and expressed in Escherichia coli as a glutathione-S-transferase-tagged fusion protein. the recombinant protein reacted with a rabbit polyclonal antibody against HSP70. Western immunoblot experiments demonstrated that sera from paracoccidioidomycosis patients recognized the purified recombinant protein, suggesting an immunological role for this protein in the infectious process. the antigenicity analysis of rHSP70 detected three internal peptides that could act as activators of T-cell proliferation.
ISSN: 1369-3786
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