Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/28153
Full metadata record
DC FieldValueLanguage
dc.contributor.authorAndrade, S. A. de
dc.contributor.authorPedrosa, MFF
dc.contributor.authorAndrade, RMG de
dc.contributor.authorOliva, MLV
dc.contributor.authorvan den Berge, C. W.
dc.contributor.authorTambourgi, D. V.
dc.date.accessioned2016-01-24T12:37:40Z-
dc.date.available2016-01-24T12:37:40Z-
dc.date.issued2005-02-04
dc.identifierhttp://dx.doi.org/10.1016/j.bbrc.2004.11.146
dc.identifier.citationBiochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 327, n. 1, p. 117-123, 2005.
dc.identifier.issn0006-291X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28153-
dc.description.abstractEnvenomation by arachnids of the genus Loxosceles can induce a variety of biological effects, including dermonecrosis and hemolysis. We have previously identified in L. intermedia venom two highly homologous proteins with sphingomyelinase activity, termed P1 and P2, responsible for all these pathological events, and also an inactive isoform P3. the toxins P1 and P2 displayed 85% identity with each other at the amino acid level and showed a 57% identity with SMase I, an active toxin from L. laeta venom. Circular dichroism was used to determine and compare the solution structure of the active and inactive isoforms. Effects of pH and temperature change on the CD spectra of the toxins were investigated and correlated with the biological activities. This study sheds new light on the structure-function relationship of homologous proteins with distinct biological properties and represents the first report on the structure-function relationship of Loxosceles sphingomyelinases D. (C) 2004 Elsevier Inc. All rights reserved.en
dc.format.extent117-123
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.rightsAcesso restrito
dc.subjectLoxoscelesen
dc.subjectvenomsen
dc.subjectcircular dichroismen
dc.subjectsphingomyelinase Den
dc.subjecthemolysisen
dc.subjectdermonecrosisen
dc.titleConformational changes of Loxosceles venom sphingomyelinases monitored by circular dichroismen
dc.typeArtigo
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionCardiff Univ
dc.description.affiliationInst Butantan, Lab Imunoquim, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Lab Bioquim, São Paulo, Brazil
dc.description.affiliationCardiff Univ, Wales Coll Med, Dept Pharmacol Toxicol & Therapeut, Cardiff, S Glam, Wales
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Lab Bioquim, São Paulo, Brazil
dc.identifier.doi10.1016/j.bbrc.2004.11.146
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000226345400019
Appears in Collections:Em verificação - Geral

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.