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dc.contributor.authorDe Angelis, Katia
dc.contributor.authorGama, V. M.
dc.contributor.authorFarah, VAM
dc.contributor.authorIrigoyen, Maria Claudia [UNIFESP]
dc.identifier.citationBrazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 38, n. 1, p. 119-125, 2005.
dc.description.abstractThe use of colored microspheres to adequately evaluate blood flow chancres under different circumstances in the same rat has been validated with a maximum of three different colors due to methodological limitations. the aim of the present study was to validate the use of four different colors measuring four repeated blood flow. changes in the same rat to assess the role of vasopressor systems in. controlling arterial pressure (AP). Red (150,000), white (200,000)), yellow (150,000), and blue (200,000) colored microspheres were infused into the left ventricle of 6 male Wistar rats 1) at rest and 2) after vasopressin (aAVP, 10 mug/kg, iv), 3) renin-angiotensin (losartan, 10 ms/kg iv), and 4) sympathetic system blockade (hexamethonium., 20 mg/kg, iv) to determine blood flow changes. AP was recorded and processed with a data acquisition system (1-kHz sampling frequency). Blood flow changes were quantified by spectrophotometry absorption peaks for colored microsphere components in the tissues evaluated. Administration of aAVP and losartan slightly reduced the AP (-5.7 +/- 0.5 and -7.8 +/- 1.2 mmHg, respectively), while hexamethonium induced a 52 +/- 3 mmHg fall in AP. the aAVP injection increased blood flow in lungs (78%), liver (117%) and skeletal muscle (>150%), while losartan administration enhanced blood flow in heart (126%), lungs (100%), kidneys (80%), and gastrocnemius (75%) and soleus (94%) muscles. Hexamethonium administration reduced only kidney blood flow (50%). in conclusion, four types of colored microspheres can be used to perform four repeated blood flow measurements in the same rat detecting small alterations such as changes in tissues with low blood flow.en
dc.publisherAssoc Bras Divulg Cientifica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsAcesso aberto
dc.subjectcolored microspheresen
dc.subjectarterial pressureen
dc.subjectsympathetic systemen
dc.subjectlocal blood flow measurementen
dc.titleBlood flow measurements in rats using four color microspheres during blockade of different vasopressor systemsen
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Santo Amaro
dc.contributor.institutionUniv Sao Judas Tadeu
dc.description.affiliationUniv São Paulo, Fac Med, Inst Coracao, Unidade Hipertensao, BR-05403000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Nefrol, São Paulo, Brazil
dc.description.affiliationUniv Santo Amaro, UNIPESQ, Santo Amaro, SP, Brazil
dc.description.affiliationUniv Sao Judas Tadeu, Lab Movimiento Humano, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Nefrol, São Paulo, Brazil
dc.description.sourceWeb of Science
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