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Title: Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
Authors: Endo, S.
Komori, T.
Ricci, Giannina [UNIFESP]
Sano, A.
Yokoyama, K.
Ohori, A.
Kamei, K.
Franco, Marcello Fabiano de [UNIFESP]
Miyaji, M.
Nishimura, K.
Chiba Univ
Fujisawa Pharmaceut Co Ltd
Universidade Federal de São Paulo (UNIFESP)
Keywords: gp43 gene
Paracoccidioides brasiliensis
Issue Date: 1-May-2004
Publisher: Elsevier B.V.
Citation: Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 234, n. 1, p. 93-97, 2004.
Abstract: Paracoccichoidomycosis is a deep mycosis Caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. the amplified DNA appeared as a ladder with a specific banding pattern. the advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
ISSN: 0378-1097
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Appears in Collections:Em verificação - Geral

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