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Title: Genotypic and phenotypic characterization of enterotoxigenic Escherichia coli (ETEC) strains isolated in Rio de Janeiro city, Brazil
Authors: Regua-Mangia, Adriana Hamond
Guth, Beatriz Ernestina Cabilio [UNIFESP]
Andrade, João Ramos da Costa
Irino, Kinue
Pacheco, Ana Beatriz Furlanetto
Ferreira, Luís Carlos de Souza
Zahner, Viviane
Teixeira, Lúcia Martins
Fdn Oowaldo Cruz
Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Universidade do Estado do Rio de Janeiro (UERJ)
Inst Adolfo Lutz Registro
Fdn Oswaldo Cruz
Keywords: enterotoxigenic Escherichia coli
molecular characterization
ETEC serotyping
cell adherence
colonization factor antigen
Issue Date: 8-Mar-2004
Publisher: Elsevier B.V.
Citation: Fems Immunology and Medical Microbiology. Amsterdam: Elsevier B.V., v. 40, n. 2, p. 155-162, 2004.
Abstract: Enterotoxigenic Escherichia coli (ETEC) strains have been implicated as important etiological agents of diarrheal disease, especially in developing countries. This group of microorganisms has been associated with a diverse range of genotypic and phenotypic markers. in the present study, 21 ETEC isolates previously defined according to the toxigenic genotypes, were characterized on the basis of O:H typing, cell adherence patterns, and colonization factors (CFs) antigens. Genetic diversity was investigated by random amplification polymorphic DNA (RAPD-PCR), pulsed-field gel electrophoresis (PFGE) and multilocus enzyme electrophoresis (MLEE). LT-I probe-positive isolates belonged to serotypes ONT:HNT, O7:H24, 048:H21, 088:H25, O148:H28, O159:H17 and 0159:H21. ST-h probe-positive isolates belonged to serotypes O159: H17, O148: H28 and O6:H-. Serotypes O148: H28, O159: H17 and O6:H- were associated with the CS6, CFA/I and CS1 CS3 antigens, respectively. Most ETEC strains exhibited a diffuse pattern of adherence to cultured epithelial cells. in general, phenotypic and genotypic characteristics correlated well. RAPD-PCR, PFGE and MLEE showed reproducibility and good discriminatory potential. the application of molecular typing systems allowed the detection of significant diversity among the isolates,. indicating a non-clonal origin and revealing intra-serotype variation overlooked by classical epidemiological approaches. the phenotypic and genotypic diversity observed lead us to recommend the use of different typing systems in order to elucidate the epidemiology of ETEC infection. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
ISSN: 0928-8244
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