Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/27290
Title: Effect of plant Kunitz inhibitors from Bauhinia bauhinioides and Bauhinia rufa on pulmonary edema caused by activated neutrophils
Authors: Neuhof, C.
Oliva, MLV
Maybauer, D.
Maybauer, M.
Oliveira, C. de
Sampaio, M. U.
Sampaio, CAM
Neuhof, H.
Univ Giessen
Universidade Federal de São Paulo (UNIFESP)
Keywords: eglin C
elastase inhibitors
isolated perfused rabbit lungs
permeability
vascular leakage
Issue Date: 1-Jun-2003
Publisher: Walter de Gruyter & Co
Citation: Biological Chemistry. Berlin: Walter de Gruyter & Co, v. 384, n. 6, p. 939-944, 2003.
Abstract: Mediators released from polymorphonuclear neutrophils, in particular elastase, are known to induce acute edematous lung injury. in this study we show that the pulmonary edema in isolated perfused rabbit lungs caused by activated neutrophils via release of elastase is significantly decreased by the Kunitztype Inhibitor BbCI (10(-5) M) from Bauhinia bauhinoides to the same degree as by eglin C (10(-5) M) from Hirudo medicinalis, which was used as a reference.The highly homologous proteinase inhibitor BrPI (10(-5) M) from Bauhinia rufa, however, did not reduce edema formation. the major difference between these inhibitors is the much higher Ki value of BrPI (K-i = 38 nM) for elastase compared to BbCI (K-i = 5.3 nM) and eglin C (K-i = 0.2 nM), respectively. Elastase liberation from activated PMNs was not influenced by the inhibitors. Our results indicate that BbCI can be a useful tool to study the role of neutrophil elastase in pathophysiological processes.
URI: http://repositorio.unifesp.br/handle/11600/27290
ISSN: 1431-6730
Other Identifiers: http://dx.doi.org/10.1515/BC.2003.105
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