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Title: High-performance liquid chromatographic separation of renin-angiotensin system peptides and most of their metabolic fragments
Authors: Pelegrini-da-Silva, A.
Prado, W. A.
Juliano, M. A.
Wilk, S.
Martins, A. R.
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Mt Sinai Sch Med
Keywords: peptides
Issue Date: 25-Nov-2002
Publisher: Elsevier B.V.
Citation: Journal of Chromatography B-analytical Technologies in the Biomedical and Life Sciences. Amsterdam: Elsevier B.V., v. 780, n. 2, p. 301-307, 2002.
Abstract: We describe here a gradient HPLC procedure for the separation, and quantification by UV absorption of renin tri- and tetradecapeptide substrates, angiotensins I, II, III, IV and V, angiotensin-derived peptides, and peptidase inhibitors including amastatin, bestatin, pepstatin, lisinopril, a renin peptide inhibitor, Z-Pro-prolinal, N-[1-(R,S)-carboxy-2-phenylethyl]-L-Ala-L-Ala-L-Phe-p-aminobenzoate, and phosphoramidon. Most peptides and peptidase inhibitors were baseline-resolved within 32 min. the overall intra- and inter-assay precisions ranged from 0.8 to 5.9 (n=6) and 2 to 13% (n=6), respectively. There was a linear relationship (correlation coefficients greater than or equal to0.9660) between peak height and peptide amount injected. in conclusion, the present method when combined with a peptidase-inhibitor paradigm can lead to the identification of renin-angiotensin system metabolizing enzymes, and when combined with radioimmunoassay can enhance the specificity of angiotensin measurement. (C) 2002 Elsevier Science B.V. All rights reserved.
ISSN: 1570-0232
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