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Title: An improved general approach for cloning and characterizing telomeres: the protozoan parasite Trypanosoma cruzi as model organism
Authors: Chiurillo, M. A.
Santos, MRM
Da Silveira, J. F.
Ramirez, J. L.
Cent Univ Venezuela
Inst Estudios Avanzados IDEA
Universidade Federal de São Paulo (UNIFESP)
Keywords: telomere organization
bacterial artificial chromosome vector-adaptor cloning of telomeres
Issue Date: 10-Jul-2002
Publisher: Elsevier B.V.
Citation: Gene. Amsterdam: Elsevier B.V., v. 294, n. 1-2, p. 197-204, 2002.
Abstract: We here describe a general strategy for cloning and characterizing telomeric and sub-telomeric regions of the human protozoan parasite Trypanosoma cruzi. the use of a bacterial artificial chromosome vector and a telomeric adaptor produced stable telomeric recombinant clones with inserts ranging from 5 to 25 kb. Analysis of these recombinants provided unique landmarks for chromosomal mapping and sequencing and enabled us to derive a more accurate picture of T cruzi telomeric organization. (C) 2002 Elsevier Science B.V. All rights reserved.
ISSN: 0378-1119
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