Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/26642
Title: The contribution of 700,000 ORF sequence tags to the definition of the human transcriptome
Authors: Camargo, A. A.
Samaia, HPB
Dias-Neto, E.
Simao, D. F.
Migotto, I. A.
Briones, Marcelo Ribeiro da Silva [UNIFESP]
Costa, F. F.
Nagai, M. A.
Verjovski-Almeida, S.
Zago, M. A.
Andrade, Luiz Eduardo Coelho [UNIFESP]
Carrer, H.
El-Dorry, HFA
Espreafico, E. M.
Habr-Gama, A.
Giannella-Neto, D.
Goldman, G. H.
Gruber, A.
Hackel, C.
Kimura, Edna Teruko [UNIFESP]
Maciel, Rui Monteiro de Barros [UNIFESP]
Marie, SKN
Martins, EAL
Nobrega, M. P.
Paco-Larson, M. L.
Pardini, MIMC
Pereira, G. G.
Pesquero, João Bosco [UNIFESP]
Rodrigues, V
Rogatto, SR
Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
Sogayar, M. C.
Sonati, MDF
Tajara, E. H.
Valentini, Sandro Roberto
Alberto, F. L.
Amaral, MEJ
Aneas, I
Arnaldi, Liliane Aparecida Teixeira [UNIFESP]
Assis, A. M. de
Bengtson, M. H.
Bergamo, N. A.
Bombonato, V
Camargo, MER de
Canevari, R. A.
Carraro, D. M.
Cerutti, Janete Maria [UNIFESP]
Correa, MLC
Correa, RFR
Costa, MCR
Curcio, C.
Hokama, POM
Ferreira, AJS
Furuzawa, Gilberto Koiti [UNIFESP]
Gushiken, T.
Ho, P. L.
Kimura, E.
Krieger, J. E.
Leite, LCC
Majumder, P.
Marins, M.
Marques, E. R.
Melo, Analy Salles de Azevedo [UNIFESP]
Melo, M.
Mestriner, Carlos Alberto
Miracca, E. C.
Miranda, D. C.
Nascimento, Ana Lucia Tabet Oller do
Nobrega, F. G.
Ojopi, EPB
Pandolfi, JRC
Pessoa, Luciana Gilbert [UNIFESP]
Prevedel, A. C.
Rahal, P.
Rainho, C. A.
Reis, EMR
Ribeiro, M. L.
Ros, N. da
Sa, R. G. de
Sales, M. M.
Sant'anna, S. C.
Santos, M. L. dos
Silva, A. M. da
Silva, Neusa Pereira da [UNIFESP]
Silva, W. A.
Silveira, R. A. da
Sousa, J. F.
Stecconi, D.
Tsukumo, F.
Valente, V
Soares, F.
Moreira, E. S.
Nunes, D. N.
Correa, R. G.
Zalcberg, H.
Carvalho, A. F.
Reis, LFL
Brentani, Ricardo Renzo [UNIFESP]
Simpson, AJG
Souza, S. J. de
Ludwig Inst Canc Res
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Fac Med Ribeirao Preto
Inst Butantan
Univ Vale Paraiba
Univ Estadual Paulista
Hosp AC Camargo Fund Antonio Prudente
Issue Date: 9-Oct-2001
Publisher: Natl Acad Sciences
Citation: Proceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 98, n. 21, p. 12103-12108, 2001.
Abstract: open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data from the central protein coding portion of transcripts. We generated a total of 696,745 ORESTES sequences from 24 human tissues and used a subset of the data that correspond to a set of 15,095 full-length mRNAs as a means of assessing the efficiency of the strategy and its potential contribution to the definition of the human transcriptome. We estimate that ORESTES sampled over 80% of all highly and moderately expressed, and between 40% and 50% of rarely expressed, human genes. in our most thoroughly sequenced tissue, the breast, the 130,000 ORESTES generated are derived from transcripts from an estimated 70% of all genes expressed in that tissue, with an equally efficient representation of both highly and poorly expressed genes. in this respect, we find that the capacity of the ORESTES strategy both for gene discovery and shotgun transcript sequence generation significantly exceeds that of conventional ESTs. the distribution of ORESTES is such that many human transcripts are now represented by a scaffold of partial sequences distributed along the length of each gene product. the experimental joining of the scaffold components, by reverse transcription-PCR, represents a direct route to transcript finishing that may represent a useful alternative to full-length cDNA cloning.
URI: http://repositorio.unifesp.br/handle/11600/26642
ISSN: 0027-8424
Other Identifiers: http://dx.doi.org/10.1073/pnas.201182798
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