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Title: Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides
Authors: Nakaie, Clovis Ryuichi [UNIFESP]
Barbosa, Simone Reis [UNIFESP]
Vieira, Renata de Freitas Fisher [UNIFESP]
Fernandez, R. M.
Cilli, Eduardo Maffud [UNIFESP]
Castrucci, Ana Maria de Lauro
Visconti, Maria Aparecida
Ito, A. S.
Lamy-Freund, M. T.
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Keywords: peptide
alpha-melanocyte stimulating hormone
electron paramagnetic resonance
2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid
spin label
Issue Date: 25-May-2001
Publisher: Elsevier B.V.
Citation: Febs Letters. Amsterdam: Elsevier B.V., v. 497, n. 2-3, p. 103-107, 2001.
Abstract: Similar to melanocyte stimulating hormone (alpha -MSH), its potent and long-acting analogue, [Nle(4), D-Phe(7)]alpha -MSH, when labeled with the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid (Toac), maintains its full biological potency, thus validating any comparative structural investigations between the two labeled peptides, Correlation times, calculated from tire electron paramagnetic resonance signal of Toac bound to the peptides, and Toac-Trp distances, estimated from the Toac fluorescence quenching of the Trp residue present in the peptides, indicate a more rigid and folded structure for the potent analogue as compared to the hormone, in aqueous medium. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
ISSN: 0014-5793
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