Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/26413
Title: Identification of human chromosome 22 transcribed sequences with ORF expressed sequence tags
Authors: Souza, Sandro José de
Camargo, Anamaria A.
Briones, Marcelo Ribeiro da Silva [UNIFESP]
Costa, Fernando Ferreira
Nagai, Maria Aparecida
Verjovski-Almeida, Sergio
Zago, Marco Antonio
Andrade, Luiz Eduardo Coelho [UNIFESP]
Carrer, Helaine
El-Dorry, Hamza FA
Espreafico, Enilza M.
Habr-Gama, Angelita
Giannella-Neto, Daniel
Goldman, Gustavo Henrique
Gruber, Arthur
Hackel, Christine
Kimura, Edna Teruko [UNIFESP]
Maciel, Rui Monteiro de Barros [UNIFESP]
Marie, Suely KN
Martins, Elizabeth AL
Nobrega, Marina P.
Paçó-Larson, Maria Luisa
Pardini, Maria Ines MC
Pereira, Gonçalo G.
Pesquero, João Bosco [UNIFESP]
Rodrigues, Vanderlei
Rogatto, Silvia R
Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
Sogayar, Mari C.
Sonati, Maria de Fátima
Tajara, Eloisa H.
Valentini, Sandro R
Acencio, Marcio
Alberto, Fernando L.
Amaral, Maria Elisabete J
Veiga, Silvio Sanches [UNIFESP]
Travassos, Luiz Rodolpho [UNIFESP]
Aneas, Ivy
Bengtson, Mario Henrique
Carraro, Dirce Maria
Carvalho, Alex Fiorini de
Carvalho, Lucia Helena
Cerutti, Janete Maria [UNIFESP]
Correa, Maria Lucia C
Costa, Maria Cristina R
Curcio, Cyntia
Gushiken, Tsieko
Ho, Paulo L.
Kimura, Elza
Leite, Luciana CC
Maia, Gustavo
Majumder, Paromita
Marins, Mozart
Matsukuma, Adriana
Melo, Analy Salles de Azevedo [UNIFESP]
Mestriner, Carlos Alberto
Miracca, Elisabete C.
Miranda, Daniela C.
Nascimento, Ana Lucia Tabet Oller do
Nobrega, Francisco G.
Ojopi, Elida PB
Pandolfi, José Rodrigo C
Pessoa, Luciana Gilbert [UNIFESP]
Rahal, Paula
Rainho, Claudia A.
Ro's, Nancy da
Sa, Renata G. de
Sales, Magaly M.
Silva, Neusa Pereira da [UNIFESP]
Silva, Tereza C.
Silva Junior, Wilson da
Simao, Daniel F.
Sousa, Josane F.
Stecconi, Daniella
Tsukumo, Fernando
Valente, Valeria
Zalcberg, Heloisa
Brentani, Ricardo Renzo [UNIFESP]
Reis, Luiz Fernando Lima
Dias-Neto, Emmanuel
Simpson, Andrew JG
Ludwig Inst Canc Res
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Ctr Biotechnol
Univ Vale do Paraiba
Univ Estadual Paulista
Issue Date: 7-Nov-2000
Publisher: Natl Acad Sciences
Citation: Proceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 97, n. 23, p. 12690-12693, 2000.
Abstract: Transcribed sequences in the human genome can be identified with confidence only by alignment with sequences derived from cDNAs synthesized from naturally occurring mRNAs. We constructed a set of 250,000 cDNAs that represent partial expressed gene sequences and that are biased toward the central coding regions of the resulting transcripts. They are termed ORF expressed sequence tags (ORESTES). the 250,000 ORESTEs were assembled into 81,429 contigs. of these, 1,181 (1.45%) were found to match sequences in chromosome 22 with at least one ORESTES contig for 162 (65.6%) of the 247 known genes, for 67 (44.6%) of the 150 related genes, and for 45 of the 148 (30.4%) EST-predicted genes on this chromosome. Using a set of stringent criteria to validate our sequences, we identified a further 219 previously unannotated transcribed sequences on chromosome 22. of these, 171 were in fact also defined by EST or full length cDNA sequences available in GenBank but not utilized in the initial annotation of the first human chromosome sequence. Thus despite representing less than 15% of all expressed human sequences in the public databases at the time of the present analysis, ORESTEs sequences defined 48 transcribed sequences on chromosome 22 not defined by other sequences. All of the transcribed sequences defined by ORESTEs coincided with DNA regions predicted as encoding exons by GENSCAN.
URI: http://repositorio.unifesp.br/handle/11600/26413
ISSN: 0027-8424
Other Identifiers: http://dx.doi.org/10.1073/pnas.97.23.12690
Appears in Collections:Em verificação - Geral

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