Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/26336
Title: DNA-based vaccination against murine paracoccidioidomycosis using the gp43 gene from Paracoccidioides brasiliensis
Authors: Pinto, A. R.
Puccia, R.
Diniz, S. N.
Franco, M. F.
Travassos, L. R.
Universidade Federal de São Paulo (UNIFESP)
Inst Adolfo Lutz
Universidade Federal de Minas Gerais (UFMG)
Keywords: Paracoccidioines brasiliensis
DNA immunisation
gp43
Issue Date: 1-Jul-2000
Publisher: Elsevier B.V.
Citation: Vaccine. Oxford: Elsevier B.V., v. 18, n. 26, p. 3050-3058, 2000.
Abstract: Gp43, the major 43-kDa antigenic glycoprotein of Paracoccidioides brasiliensis, or its 15-amino acid inner peptide (P10), induces a T-CD4(+), Th1 cellular immune response which protects BALB/c mice from intratracheal infection by virulent yeast forms. We investigated whether DNA vaccination using the gp43 gene could elicit protective immunity against P. brasiliensis. Animals immunised intramuscularly (i.m.) or intradermally (i.d.) with plasmid DNA containing the gp43 gene induced a specific, long lasting humoral and cellular immune response. A mixed Th1/Th2 cellular immune response in DNA-immunized mice was modulated in vivo by IFN-gamma and was protective in BALB/c mice. A significant decrease in the lung colony forming units (CFUs) and reduced, or no dissemination to the spleen and liver of immunised mice were observed. (C) 2000 Elsevier B.V. All rights reserved.
URI: http://repositorio.unifesp.br/handle/11600/26336
ISSN: 0264-410X
Other Identifiers: http://dx.doi.org/10.1016/S0264-410X(00)00074-8
Appears in Collections:Em verificação - Geral

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