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|Title:||Identification of sialic acids on the cell surface of Candida albicans|
Alviano, D. S.
Alviano, C. S.
Travassos, L. R.
Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
|Citation:||Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1474, n. 2, p. 262-268, 2000.|
|Abstract:||The cell-surface expression of sialic acids in two isolates of Candida albicans was analyzed by thin-layer and gas chromatography, binding of lectins, colorimetry, sialidase treatment and flow cytofluorimetry with fluorescein-labeled lectins. N-acetylneuraminic acid (NANA) was the only derivative found in both strains of C. albicans grown in a chemically defined medium. Its identification was confirmed by mass spectrometry in comparison with an authentic standard. the density of sialic acid residues per cell ranged from 1.6x10(6) to 2.8x10(6). the surface distribution of sialic acids over the entire C. albicans was inferred from labeling with fluorescein-Limulus polyphemus and Limax flavus agglutinins and directly observed by optical microscopy with (FITC)-Sambucus nigra agglutinin (SNA), abrogated by previous treatment of yeasts with bacterial sialidase. Sialidase-treated yeasts generated beta-galactopyranosyl terminal residues that reacted with peanut agglutinin. in C. albicans N-acetyl-neuraminic acids are alpha 2,6- and alpha 2,3-linked as indicated by yeast binding to SNA and Maackia amurensis agglutinin. the alpha 2,6-linkage clearly predominated in both strains. We also investigated the contribution of sialic acids to the electronegativity of C. albicans, an important factor determining fungal interactions in vivo. Adhesion of yeast cells to a cationic solid phase substrate (poly-L-lysine) was mediated in part by sialic acids, since the number of adherent cells was significantly reduced after treatment with bacterial sialidase. the present evidence adds C. albicans to the list of pathogenic Fungi that synthesize sialic acids, which contribute to the negative charge of fungal cells and have a role in their specific interaction with the host tissue. (C) 2000 Elsevier Science B.V. All rights reserved.|
|Appears in Collections:||Em verificação - Geral|
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