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Title: Content of liver and brain ubiquinol-9 and ubiquinol-10 after chronic ethanol intake in rats subjected to two levels of dietary alpha-tocopherol
Authors: Junqueira, VBC
Carrasquedo, F.
Azzalis, L. A.
Giavarotti, KAS
Giavarotti, L.
Rodrigues, L.
Fraga, C. G.
Boveris, A.
Videla, L. A.
Universidade Federal de São Paulo (UNIFESP)
Univ Buenos Aires
Universidade de São Paulo (USP)
Univ Chile
Keywords: chronic ethanol ingestion
total glutathione
Issue Date: 1-Jan-2000
Publisher: Harwood Acad Publ Gmbh
Citation: Free Radical Research. Reading: Harwood Acad Publ Gmbh, v. 33, n. 3, p. 313-319, 2000.
Abstract: To assess the effect of chronic ethanol ingestion in the content of the reduced forms of coenzymes Q(9) (ubiquinol-9) and Q(10) (ubiquinol-10) as a factor contributing to oxidative stress in liver and brain, male Wistar rats were fed ad libitum a basal diet containing either 10 or 2.5 mg alpha-tocopherol/100% diet (controls), or the same basal diet plus a 32% ethanol-25% sucrose solution. After three months treatment, ethanol chronically-treated rats showed identical growth rates to the isocalorically pair-fed controls, irrespectively of alpha-tocopherol dietary level. Lowering dietary alpha-tocopherol led to a decreased content of this vitamin in the liver and brain of control rats, without changes in that of ubiquinol-9, and increased levels of hepatic ubiquinol-10 and total glutathione (tGSH), accompanied by a decrease in brain tGSH. At the two levels of dietary alpha-tocopherol, ethanol treatment significantly decreased the content of hepatic alpha-tocopherol and ubiquinols 9 and 10. This effect was significantly greater at 10 mg alpha-tocopherol/100% diet than at 2.5, whereas those of tGSH were significantly elevated by 43% and 9%, respectively. Chronic ethanol intake did not alter the content of brain alpha-tocopherol and tGSH, whereas those of ubiquinol-9 were significantly lowered by 20% and 14% in rats subjected to 10 and 2.5 mg alpha-tocopherol/100 g diet, respectively. It is concluded that chronic ethanol intake at two levels of dietary alpha-tocopherol induces a depletion of hepatic alpha-tocopherol and ubiquinols 9 and 10, thus contributing to ethanol-induced oxidative stress in the liver tissue. This effect of ethanol is dependent upon the dietary level of alpha-tocopherol, involves a compensatory enhancement in hepatic tGSH availability, and is not observed in the brain tissue, probably due to its limited capacity for ethanol biotransformation and glutathione synthesis.
ISSN: 1071-5762
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