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dc.contributor.authorDong, X. W.
dc.contributor.authorWang, J. S.
dc.contributor.authorKabir, F. N.
dc.contributor.authorShaw, M.
dc.contributor.authorReed, A. M.
dc.contributor.authorStein, L.
dc.contributor.authorAndrade, Luiz Eduardo Coelho [UNIFESP]
dc.contributor.authorTrevisani, Virgínia Fernandes Moça [UNIFESP]
dc.contributor.authorMiller, M. L.
dc.contributor.authorFujii, T.
dc.contributor.authorAkizuki, M.
dc.contributor.authorPachman, L. M.
dc.contributor.authorSatoh, M.
dc.contributor.authorReeves, W. H.
dc.identifier.citationArthritis and Rheumatism. Philadelphia: Lippincott Williams & Wilkins, v. 43, n. 1, p. 85-93, 2000.
dc.description.abstractObjective. To evaluate the specificity of anti-DEK antibodies for juvenile rheumatoid arthritis (JRA),Methods. Anti-DEK autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) using affinity-purified his6-DEK fusion protein. Sera from 639 subjects (417 patients with systemic autoimmune disease, 13 with sarcoidosis, 44 with pulmonary tuberculosis, 125 with uveitis, and 6 with scleritis, and 34 healthy control subjects) were screened. Reactivity was verified by immunoblotting and immunoprecipitation Studies using baculovirus-expressed human DEK.Results. Anti-DEK activity was found at the following frequencies: JRA 39.4% (n = 71), systemic lupus erythematosus (SLE) 25.1% (n = 216), sarcoidosis 46.2% (n = 13), rheumatoid arthritis 15.5% (n = 71), systemic sclerosis 36.0% (n = 22), polymyositis 6.2% (n = 16), and adult Still's disease 0% (n = 21), Autoantibodies also were detected in 9.1% of tuberculosis sera (n = 44), but were undetectable in sera from the 34 healthy controls. Western blot and immunoprecipitation assay results correlated well with the ELISA findings. in general, levels of anti-DEK autoantibodies were higher in SLE than in other patient subsets, including JRA.Conclusion. Anti-DEK autoantibodies are less specific for JRA than previously believed. They are produced in association with a variety of inflammatory conditions, many of which are associated with granuloma formation and/or predominant Th1 cytokine production. Anti-DEK antibodies may be a marker for a subset of autoimmunity associated with interferon-gamma production rather than a particular disease subset.en
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofArthritis and Rheumatism
dc.rightsAcesso aberto
dc.titleAutoantibodies to DEK oncoprotein in human inflammatory diseaseen
dc.contributor.institutionUniv N Carolina
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionNorthwestern Univ
dc.contributor.institutionKeio Univ Hosp
dc.description.affiliationUniv N Carolina, Thurston Arthrit Res Ctr, Chapel Hill, NC USA
dc.description.affiliationUniv N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA
dc.description.affiliationUniversidade Federal de São Paulo, São Paulo, Brazil
dc.description.affiliationNorthwestern Univ, Sch Med, Chicago, IL USA
dc.description.affiliationKeio Univ Hosp, Tokyo, Japan
dc.description.affiliationUnifespUniversidade Federal de São Paulo, São Paulo, Brazil
dc.description.sourceWeb of Science
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