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Title: An improved method to distinguish Entamoeba histolytica and Entamoeba dispar
Authors: Gomes, M. A.
Pesquero, J. B.
Furst, C.
Valle, P. R.
Pesquero, J. L.
Silva, E. F.
Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
Keywords: Entamoeba histolytica
Entamoeba dispar differentiation
DNA electrophoresis
DNA conformation
Issue Date: 1-Oct-1999
Publisher: Cambridge Univ Press
Citation: Parasitology. New York: Cambridge Univ Press, v. 119, p. 359-362, 1999.
Abstract: A 482 base pair gene fragment from samples of amoebae E. histolytica and E. dispar was amplified by PCR. the amplification products of fragments from the 2 species of amoebae presented differences in mobility in non-denaturing polyacrylamide gel, probably due to sequence-dependent conformational alterations in the DNA fragments. the method described here permits E. histolytica and E. dispar to be distinguished with greater sensitivity and rapidity.
ISSN: 0031-1820
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Appears in Collections:Em verificação - Geral

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