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dc.contributor.authorMacchione, Mariangela
dc.contributor.authorOliveira, Alexandre P.
dc.contributor.authorGallafrio, Cristina T.
dc.contributor.authorMuchão, Fabio B.
dc.contributor.authorObara, Marcos T.
dc.contributor.authorGuimarães, Eliane T.
dc.contributor.authorArtaxo, Paulo
dc.contributor.authorKing, Malcolm
dc.contributor.authorLorenzi-Filho, Geraldo
dc.contributor.authorJunqueira, Virginia Berlanga Campos [UNIFESP]
dc.contributor.authorSaldiva, Paulo Hilário Nascimento
dc.identifier.citationEnvironmental Health Perspectives. Res Triangle Pk: Us Dept Health Human Sciences Public Health Science, v. 107, n. 10, p. 829-833, 1999.
dc.description.abstractThis work was designed to evaluate the toxicity of inhalable particles less than or equal to 10 pm in aerodynamic diameter (PM10) collected from the urban air in São Paulo, Brazil, to the mucociliary apparatus using the frog palate preparation. Seven groups of frog palates were immersed in different concentrations of PM10 diluted in Ringer's solution during 120 min: 0 (control, n = 31); 50 (n = 10); 100 (n = 9); 500 (n = 28); 1,000 (n = 10); 5,000 (n = 11); and 10,000 mu g/m(3) (n = 10). Mucociliary transport and transepithelial potential difference were determined at 0, 30, 60, and 120 min exposure. Additional groups (control and 500 pg/m(3)) were studied by means of morphometric analyses (quantification of the amount of intraepithelial and surface mucins), measurement of cilia beat frequency, and quantification of total glutathione. Mucociliary transport and transepithelial potential difference were significantly decreased at higher concentrations of PM10 (p = 0.03 and p = 0.02, respectively). Exposure to PM10 also elicited a significant decrease of total glutathione (p = 0.003) and depletion of neutral intraepithelial mucins (p = 0.0461). These results show that PM10 can promote significant alterations in ciliated epithelium in vitro.en
dc.publisherUs Dept Health Human Sciences Public Health Science
dc.relation.ispartofEnvironmental Health Perspectives
dc.rightsAcesso aberto
dc.subjectair pollutionen
dc.subjectcilia beat frequencyen
dc.subjectmucociliary transporten
dc.subjectmorphometric analysesen
dc.titleAcute effects of inhalable particles on the frog palate mucociliary epitheliumen
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Alberta
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniv São Paulo, Sch Med, Fac Med, Lab Poluicao Atmosfer Expt, BR-01246903 São Paulo, Brazil
dc.description.affiliationHC FMUSP, Inst Coracao, São Paulo, Brazil
dc.description.affiliationUSP, Dept Bioquim, Inst Quim, BR-09500900 São Paulo, Brazil
dc.description.affiliationUSP, Inst Fis, Grp Estudos Poluicao Ar, BR-09500900 São Paulo, Brazil
dc.description.affiliationUniv Alberta, Dept Med, Pulm Res Grp, Edmonton, AB, Canada
dc.description.affiliationUNIFESP, Ctr Estudios Envelhecimento, São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Ctr Estudios Envelhecimento, São Paulo, Brazil
dc.description.sourceWeb of Science
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