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|Title:||Acute effects of inhalable particles on the frog palate mucociliary epithelium|
Oliveira, Alexandre P.
Gallafrio, Cristina T.
Muchão, Fabio B.
Obara, Marcos T.
Guimarães, Eliane T.
Junqueira, Virginia Berlanga Campos [UNIFESP]
Saldiva, Paulo Hilário Nascimento
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
cilia beat frequency
|Publisher:||Us Dept Health Human Sciences Public Health Science|
|Citation:||Environmental Health Perspectives. Res Triangle Pk: Us Dept Health Human Sciences Public Health Science, v. 107, n. 10, p. 829-833, 1999.|
|Abstract:||This work was designed to evaluate the toxicity of inhalable particles less than or equal to 10 pm in aerodynamic diameter (PM10) collected from the urban air in São Paulo, Brazil, to the mucociliary apparatus using the frog palate preparation. Seven groups of frog palates were immersed in different concentrations of PM10 diluted in Ringer's solution during 120 min: 0 (control, n = 31); 50 (n = 10); 100 (n = 9); 500 (n = 28); 1,000 (n = 10); 5,000 (n = 11); and 10,000 mu g/m(3) (n = 10). Mucociliary transport and transepithelial potential difference were determined at 0, 30, 60, and 120 min exposure. Additional groups (control and 500 pg/m(3)) were studied by means of morphometric analyses (quantification of the amount of intraepithelial and surface mucins), measurement of cilia beat frequency, and quantification of total glutathione. Mucociliary transport and transepithelial potential difference were significantly decreased at higher concentrations of PM10 (p = 0.03 and p = 0.02, respectively). Exposure to PM10 also elicited a significant decrease of total glutathione (p = 0.003) and depletion of neutral intraepithelial mucins (p = 0.0461). These results show that PM10 can promote significant alterations in ciliated epithelium in vitro.|
|Appears in Collections:||Em verificação - Geral|
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