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Title: Kininogenase activity by the major cysteinyl proteinase (Cruzipain) from Trypanosoma cruzi
Authors: Del Nery, Elaine [UNIFESP]
Juliano, Maria Aparecida [UNIFESP]
Lima, Ana Paula CA
Scharfstein, Julio
Juliano, Luiz [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
Issue Date: 10-Oct-1997
Publisher: Amer Soc Biochemistry Molecular Biology Inc
Citation: Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 272, n. 41, p. 25713-25718, 1997.
Abstract: The major isoform of Trypanosona cruzi cysteinyl proteinase (cruzipain) has generated Lys-bradykinin (Lys-BK or kallidin), a proinflammatory peptide, by proteolysis of kininogen. the releasing of this peptide was demonstrated by mass spectrometry, radioimmunoassay, and ileum contractile responses, the kinin-releasing activity was immunoabsorbed selectively by monoclonal antibodies to the characteristic COOH-terminal domain of cruzipain. To determine the hydrolysis steps that account for the kininogenase activity of cruzipain, we synthesized a fluorogenic peptide (o-aminobenzoyl-Leu-Gly-Met-Ile-Ser-Leu-Met-Lys-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg(389)-Ser(390)-Ser-Arg-Ile-NH2) based on the sequence Leu(373) to Ile(393) of the human high molecular weight kininogen, the hydrolysis products from this peptide were isolated by high performance liquid chromatography, and Lys-BK was characterized as the major released kinin by mass spectrometry. Intramolecularly quenched fluorogenic peptides spanning the Met(379)-Lys(380) and Arg(389)-Ser(390) bradykinin-flanking sequences were then used to assess the substrate specificity re requirements of the parasite-derived protease compared with two COOH-terminal truncated recombinant isoforms (cruzain and cruzipain 2). in contrast to the high catalytic efficiency of parasite-derived cruzipain, the recombinant proteinases cleaved the bradykinin-flanking sites at markedly different rates. in addition, we also demonstrated that cruzipain activates plasmatic prekallikrein, which would be a second and indirect way of the parasite protease to release bradykinin.
ISSN: 0021-9258
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Appears in Collections:Em verificação - Geral

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