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Title: Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD
Authors: Pacheco, Ana Beatriz Furlanetto
Guth, Beatriz Ernestina Cabilio [UNIFESP]
Soares, Kellen C C
Almeida, Darcy Fontoura de
Ferreira, Luís Carlos de Souza
Universidade Federal de São Paulo (UNIFESP)
Keywords: Escherichia coli
RAPD typing
clonal grouping
Issue Date: 15-Mar-1997
Publisher: Elsevier B.V.
Citation: Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 148, n. 2, p. 255-260, 1997.
Abstract: The genetic diversity in a group of Escherichia coli strains belonging to serogroup O6 but expressing different H antigens was investigated by random amplification of polymorphic DNA (RAPD). Isolates of serotypes H16, H1, H31, and non-motile (NM) strains were typed using a set of 3 primers with different G+C contents. the amplified band arrays allowed the identification of 3 main clonal clusters corresponding to each O:H serotype analyzed. Based on their RAPD profiles NEVI strains could be assigned to either H1 or H31 serotypes. the results indicate that the flagellar antigen and the RAPD fingerprint represent reliable clonal markers in this E. coli group.
ISSN: 0378-1097
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Appears in Collections:Em verificação - Geral

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