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|Title:||GENES-CODING for SHIGA-LIKE TOXINS in BOVINE VEROTOXIN-PRODUCING ESCHERICHIA-COLI (VTEC) STRAINS BELONGING TO DIFFERENT O-K-H SEROTYPES|
Blanco, J. E.
Gonzalez, E. A.
Zerbini, L. F.
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
|Keywords:||ESCHERICHIA COLI, GENETICS|
|Citation:||Veterinary Microbiology. Amsterdam: Elsevier B.V., v. 42, n. 2-3, p. 105-110, 1994.|
|Abstract:||Forty-six verotoxin-producing Escherichia coli (VTEC) strains isolated from diarrhoeic and healthy calves in Spain were examined for DNA sequences homologous to genes for verotoxins (VT1 and VT2) and enterotoxins (LT-I, LT-II, STaH, STaP and STb). Hybridisation showed that 26 (57%) of VTEC strains carried VT1 genes, 13 (28%) possessed VT2 genes, and 7 (15%) carried both VT1 and VT2 genes. No VTEC strains hybridised with DNA probes for enterotoxins. A correlation was found between the serotype and type of VT produced. Thus, all strains of serotypes 026:K-:H11 (13 strains), 0103:K-:H2 (3 strains) and 0128:K?:H- (4 strains) hybridised with the VT1 probe only, whereas all strains of serotypes 03:K-:H4 (3 strains) and 0113:K-:H21 (4 strains) were positive with the VT2 probe only. By contrast, 081:K?:H28 (2 strains) and O157:K-:H- (2 strains) strains hybridised with both VT1 and VT2 probes. One strain of serotype O157:K-:H7 was VT2 positive.|
|Appears in Collections:||Em verificação - Geral|
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