Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/25443
Title: GENES-CODING for SHIGA-LIKE TOXINS in BOVINE VEROTOXIN-PRODUCING ESCHERICHIA-COLI (VTEC) STRAINS BELONGING TO DIFFERENT O-K-H SEROTYPES
Authors: Blanco, M.
Blanco, J.
Blanco, J. E.
Gonzalez, E. A.
Gomes, TAT
Zerbini, L. F.
Yano, T.
Decastro, AFP
UNIV SANTIAGO
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Keywords: ESCHERICHIA COLI, GENETICS
DNA PROBE
PATHOGENICITY, BACTERIA
TOXIN
VT GENES
Issue Date: 1-Nov-1994
Publisher: Elsevier B.V.
Citation: Veterinary Microbiology. Amsterdam: Elsevier B.V., v. 42, n. 2-3, p. 105-110, 1994.
Abstract: Forty-six verotoxin-producing Escherichia coli (VTEC) strains isolated from diarrhoeic and healthy calves in Spain were examined for DNA sequences homologous to genes for verotoxins (VT1 and VT2) and enterotoxins (LT-I, LT-II, STaH, STaP and STb). Hybridisation showed that 26 (57%) of VTEC strains carried VT1 genes, 13 (28%) possessed VT2 genes, and 7 (15%) carried both VT1 and VT2 genes. No VTEC strains hybridised with DNA probes for enterotoxins. A correlation was found between the serotype and type of VT produced. Thus, all strains of serotypes 026:K-:H11 (13 strains), 0103:K-:H2 (3 strains) and 0128:K?:H- (4 strains) hybridised with the VT1 probe only, whereas all strains of serotypes 03:K-:H4 (3 strains) and 0113:K-:H21 (4 strains) were positive with the VT2 probe only. By contrast, 081:K?:H28 (2 strains) and O157:K-:H- (2 strains) strains hybridised with both VT1 and VT2 probes. One strain of serotype O157:K-:H7 was VT2 positive.
URI: http://repositorio.unifesp.br/handle/11600/25443
ISSN: 0378-1135
Other Identifiers: http://dx.doi.org/10.1016/0378-1135(94)90010-8
Appears in Collections:Em verificação - Geral

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