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|Title:||GLYCOCONJUGATES of TRYPANOSOMA-CRUZI - A 74 KD ANTIGEN of TRYPOMASTIGOTES SPECIFICALLY REACTS WITH LYTIC ANTI-ALPHA-GALACTOSYL ANTIBODIES FROM PATIENTS WITH CHRONIC CHAGAS-DISEASE|
|Authors:||Almeida, I. C.|
Krautz, G. M.
Krettli, A. U.
Travassos, L. R.
Universidade Federal de São Paulo (UNIFESP)
FUNDACAO OSWALDO CRUZ
|Citation:||Journal of Clinical Laboratory Analysis. New York: Wiley-liss, v. 7, n. 6, p. 307-316, 1993.|
|Abstract:||Protective, lytic antibodies are believed to be correlated with active Trypanosoma cruzi infection. in patients with chronic infection, antibodies lysing trypomastigote forms recognize chiefly alpha-galactosyl structures at the parasite surface. the target molecules on cell-derived trypomastigotes that react with anti-alpha-galactosyl antibodies (anti-Gal) from patients with chronic Chagas disease were investigated. Glycoconjugates were isolated from trypomastigotes and shown to absorb purified Chagasic (Ch) anti-Gal effectively as well as lytic antibodies from Ch sera. Active fractions were F2 (74 kD and 95.6 kD) and F3 (120-200 kD). A differential reactivity with antibodies from untreated Ch patients (trypanolytic) and from treated. presumably cured. individuals (not trypanolytic) was evident using F2 and F3 antigenic fractions. No cross-reactivity with heterologous sera (other infections) was observed. the F2 glycoconjugate (mostly 74 kD) can be used in the diagnosis of active Chagas infection, replacing the quantitative determination of complement-mediated lysis. With the present sample of patients' sera and normal human sera, it showed 100% sensitivity and specificity. (C) 1993 Wiley-Liss. Inc.|
|Appears in Collections:||Em verificação - Geral|
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