Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/25295
Title: GLYCOCONJUGATES of TRYPANOSOMA-CRUZI - A 74 KD ANTIGEN of TRYPOMASTIGOTES SPECIFICALLY REACTS WITH LYTIC ANTI-ALPHA-GALACTOSYL ANTIBODIES FROM PATIENTS WITH CHRONIC CHAGAS-DISEASE
Authors: Almeida, I. C.
Krautz, G. M.
Krettli, A. U.
Travassos, L. R.
Universidade Federal de São Paulo (UNIFESP)
FUNDACAO OSWALDO CRUZ
Keywords: AMERICAN TRYPANOSOMIASIS
ANTI-GAL ANTIBODY
IMMUNOLOGICAL DIAGNOSIS
DRUG-TREATED PATIENTS
TRYPANOLYSIS
CHEMOLUMINESCENCE
Issue Date: 1-Jan-1993
Publisher: Wiley-Blackwell
Citation: Journal of Clinical Laboratory Analysis. New York: Wiley-liss, v. 7, n. 6, p. 307-316, 1993.
Abstract: Protective, lytic antibodies are believed to be correlated with active Trypanosoma cruzi infection. in patients with chronic infection, antibodies lysing trypomastigote forms recognize chiefly alpha-galactosyl structures at the parasite surface. the target molecules on cell-derived trypomastigotes that react with anti-alpha-galactosyl antibodies (anti-Gal) from patients with chronic Chagas disease were investigated. Glycoconjugates were isolated from trypomastigotes and shown to absorb purified Chagasic (Ch) anti-Gal effectively as well as lytic antibodies from Ch sera. Active fractions were F2 (74 kD and 95.6 kD) and F3 (120-200 kD). A differential reactivity with antibodies from untreated Ch patients (trypanolytic) and from treated. presumably cured. individuals (not trypanolytic) was evident using F2 and F3 antigenic fractions. No cross-reactivity with heterologous sera (other infections) was observed. the F2 glycoconjugate (mostly 74 kD) can be used in the diagnosis of active Chagas infection, replacing the quantitative determination of complement-mediated lysis. With the present sample of patients' sera and normal human sera, it showed 100% sensitivity and specificity. (C) 1993 Wiley-Liss. Inc.
URI: http://repositorio.unifesp.br/handle/11600/25295
ISSN: 0887-8013
Other Identifiers: http://dx.doi.org/10.1002/jcla.1860070603
Appears in Collections:Em verificação - Geral

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