Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/25228
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dc.contributor.authorFerro, E. S.
dc.contributor.authorHamassaki, D. E.
dc.contributor.authorCamargo, ACM
dc.contributor.authorBritto, LRG
dc.date.accessioned2016-01-24T11:40:05Z-
dc.date.available2016-01-24T11:40:05Z-
dc.date.issued1991-11-01
dc.identifierhttp://dx.doi.org/10.1111/j.1471-4159.1991.tb06363.x
dc.identifier.citationJournal of Neurochemistry. Philadelphia: Lippincott-raven Publ, v. 57, n. 5, p. 1643-1649, 1991.
dc.identifier.issn0022-3042
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/25228-
dc.description.abstractEndo-oligopeptidase A, EC 3.4.22.19, converts small enkephalin-containing peptides into the corresponding enkephalins in vitro. We investigated the presence of endooligopeptidase A in the retina and its possible colocalization with enkephalins in retinal neurons. the specific activity of endo-oligopeptidase A found in pigeon retinae (30.3 +/- 7.3 mU/mg, mean +/- standard deviation) was four times higher than in rabbit retinae (7.0 +/- 1.1 mU/mg). the enzyme activity was not modified by EDTA, but it was enhanced by dithiothreitol and inhibited by zinc and 5,5'-dithiobis(2-nitrobenzoic acid). Immunohistochemical experiments with a purified antiserum against rabbit endo-oligopeptidase A revealed labeled neurons in both the inner nuclear layer and the ganglion cell layer of pigeon and rabbit retinae. Double-labeling immunofluorescence experiments demonstrated that about 90% of neurons containing endo-oligopeptidase A-like immunoreactivity also contained [Leu5]-enkephalin-like immunoreactivity. These colocalization results may represent an important step toward the demonstration of the possible involvement of endo-oligopeptidase A in enkephalin generation in vivo.en
dc.format.extent1643-1649
dc.language.isoeng
dc.publisherLippincott-raven Publ
dc.relation.ispartofJournal of Neurochemistry
dc.rightsAcesso restrito
dc.subjectENDO-OLIGOPEPTIDASE-Aen
dc.subjectENDOPEPTIDASE 22.19en
dc.subjectENKEPHALINSen
dc.subjectOPIOID PEPTIDESen
dc.subjectRETINAL NEUROPEPTIDESen
dc.titleENDO-OLIGOPEPTIDASE-A, A PUTATIVE ENKEPHALIN-GENERATING ENZYME, in the VERTEBRATE RETINAen
dc.typeArtigo
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionSão Paulo STATE UNIV
dc.description.affiliationESCOLA PAULISTA MED SCH,DEPT BIOPHYS,BR-04023 São Paulo,BRAZIL
dc.description.affiliationSão Paulo STATE UNIV,INST BIOMED SCI,DEPT PHYSIOL & BIOPHYS,BR-05508 São Paulo,BRAZIL
dc.description.affiliationUnifespESCOLA PAULISTA MED SCH,DEPT BIOPHYS,BR-04023 São Paulo,BRAZIL
dc.identifier.doi10.1111/j.1471-4159.1991.tb06363.x
dc.description.sourceWeb of Science
dc.identifier.wosWOS:A1991GL28800025
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