Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/25228
Title: ENDO-OLIGOPEPTIDASE-A, A PUTATIVE ENKEPHALIN-GENERATING ENZYME, in the VERTEBRATE RETINA
Authors: Ferro, E. S.
Hamassaki, D. E.
Camargo, ACM
Britto, LRG
Universidade Federal de São Paulo (UNIFESP)
São Paulo STATE UNIV
Keywords: ENDO-OLIGOPEPTIDASE-A
ENDOPEPTIDASE 22.19
ENKEPHALINS
OPIOID PEPTIDES
RETINAL NEUROPEPTIDES
Issue Date: 1-Nov-1991
Publisher: Lippincott-raven Publ
Citation: Journal of Neurochemistry. Philadelphia: Lippincott-raven Publ, v. 57, n. 5, p. 1643-1649, 1991.
Abstract: Endo-oligopeptidase A, EC 3.4.22.19, converts small enkephalin-containing peptides into the corresponding enkephalins in vitro. We investigated the presence of endooligopeptidase A in the retina and its possible colocalization with enkephalins in retinal neurons. the specific activity of endo-oligopeptidase A found in pigeon retinae (30.3 +/- 7.3 mU/mg, mean +/- standard deviation) was four times higher than in rabbit retinae (7.0 +/- 1.1 mU/mg). the enzyme activity was not modified by EDTA, but it was enhanced by dithiothreitol and inhibited by zinc and 5,5'-dithiobis(2-nitrobenzoic acid). Immunohistochemical experiments with a purified antiserum against rabbit endo-oligopeptidase A revealed labeled neurons in both the inner nuclear layer and the ganglion cell layer of pigeon and rabbit retinae. Double-labeling immunofluorescence experiments demonstrated that about 90% of neurons containing endo-oligopeptidase A-like immunoreactivity also contained [Leu5]-enkephalin-like immunoreactivity. These colocalization results may represent an important step toward the demonstration of the possible involvement of endo-oligopeptidase A in enkephalin generation in vivo.
URI: http://repositorio.unifesp.br/handle/11600/25228
ISSN: 0022-3042
Other Identifiers: http://dx.doi.org/10.1111/j.1471-4159.1991.tb06363.x
Appears in Collections:Em verificação - Geral

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