Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/25177
Title: PURIFICATION of SOLUBLE HUMAN LYMPHOCYTE-T RECEPTORS for SHEEP ERYTHROCYTES
Authors: Itano, E. N.
Ono, M. A.
Sumigawa, M.
Longo, I. M.
Moura, N. C.
Mendes, N. F.
Universidade Federal de São Paulo (UNIFESP)
Keywords: E-RECEPTORS
CD2
CHROMATOGRAPHY
Issue Date: 1-Jan-1991
Publisher: Wiley-Blackwell
Citation: Journal of Clinical Laboratory Analysis. New York: Wiley-liss, v. 5, n. 3, p. 162-167, 1991.
Abstract: Using a polyclonal heterologous anti-soluble E-receptor serum, we identified molecules of molecular weight circa 58,000 and 150,000.The soluble receptor molecule with molecular weight of approximately 58,000 (R(s)1) was initially purified from supernatant of heated lymphocytes through chromatography on Sephadex G-200 and/or DEAE-cellulose. the soluble receptor molecule with molecular weight of approximately 150,000 (R(s)2) is detected at high levels in the serum of patients with cancer and uremia. R(s)1 and R(s)2 present in serum from cancer patients were purified by chromatography on Sephadex G-200 and by affinity chromatography using anti-R(s)1 IgG.I-131-labelled supernatant of heated lymphocytes binds to sheep erythrocytes and the elution and analysis of the molecules adsorbed showed bands of molecular weights approximately 58,000 and 150,000, confirming the receptor activity of these molecules.
URI: http://repositorio.unifesp.br/handle/11600/25177
ISSN: 0887-8013
Other Identifiers: http://dx.doi.org/10.1002/jcla.1860050303
Appears in Collections:Em verificação - Geral

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.