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|Title:||PURIFICATION of SOLUBLE HUMAN LYMPHOCYTE-T RECEPTORS for SHEEP ERYTHROCYTES|
|Authors:||Itano, E. N.|
Ono, M. A.
Longo, I. M.
Moura, N. C.
Mendes, N. F.
Universidade Federal de São Paulo (UNIFESP)
|Citation:||Journal of Clinical Laboratory Analysis. New York: Wiley-liss, v. 5, n. 3, p. 162-167, 1991.|
|Abstract:||Using a polyclonal heterologous anti-soluble E-receptor serum, we identified molecules of molecular weight circa 58,000 and 150,000.The soluble receptor molecule with molecular weight of approximately 58,000 (R(s)1) was initially purified from supernatant of heated lymphocytes through chromatography on Sephadex G-200 and/or DEAE-cellulose. the soluble receptor molecule with molecular weight of approximately 150,000 (R(s)2) is detected at high levels in the serum of patients with cancer and uremia. R(s)1 and R(s)2 present in serum from cancer patients were purified by chromatography on Sephadex G-200 and by affinity chromatography using anti-R(s)1 IgG.I-131-labelled supernatant of heated lymphocytes binds to sheep erythrocytes and the elution and analysis of the molecules adsorbed showed bands of molecular weights approximately 58,000 and 150,000, confirming the receptor activity of these molecules.|
|Appears in Collections:||Em verificação - Geral|
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