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Title: Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor
Authors: Lima, Eleonidas Moura
Rissino, Jorge Dores
Harada, Maria Lucia
Assumpção, Paulo Pimentel [UNIFESP]
Demachki, Samia
Guimarães, Adriana Costa
Casartelli, Carla [UNIFESP]
Smith, Marilia de Arruda Cardoso [UNIFESP]
Burbano, Rommel Rodríguez [UNIFESP]
Universidade de São Paulo (USP)
Universidade Federal do Pará Centro de Ciências Biológicas Departamento de Biologia
Universidade Federal do Pará Centro de Ciências Biológicas Departamento de Genética
Universidade Federal do Pará Hospital Universitário João de Barros Barreto Departamento de Patologia e Serviço de Cirurgia
Universidade Federal de São Paulo (UNIFESP)
Keywords: Cell line ACP01
Human gastric adenocarcinoma
In vitro immortalization
Chromosome 8 trisomy
Clonal expansion
Issue Date: 1-Dec-2004
Publisher: Associação Brasileira de Divulgação Científica
Citation: Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 12, p. 1831-1838, 2004.
Abstract: Gastric cancer is the second most frequent type of neoplasia and also the second most important cause of death in the world. Virtually all the established cell lines of gastric neoplasia were developed in Asian countries, and western countries have contributed very little to this area. In the present study we describe the establishment of the cell line ACP01 and characterize it cytogenetically by means of in vitro immortalization. Cells were transformed from an intestinal-type gastric adenocarcinoma (T4N2M0) originating from a 48-year-old male patient. This is the first gastric adenocarcinoma cell line established in Brazil. The most powerful application of the cell line ACP01 is in the assessment of cytotoxicity. Solid tumor cell lines from different origins have been treated with several conventional and investigational anticancer drugs. The ACP01 cell line is triploid, grows as a single, non-organized layer, similar to fibroblasts, with focus formation, heterogeneous division, and a cell cycle of approximately 40 h. Chromosome 8 trisomy, present in 60% of the cells, was the most frequent cytogenetic alteration. These data lead us to propose a multifactorial triggering of gastric cancer which evolves over multiple stages involving progressive genetic changes and clonal expansion.
ISSN: 0100-879X
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