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Title: HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
Authors: Rossini, Marília Almeida Antunes
Diaz, Ricardo Sobhie [UNIFESP]
Caseiro, Marcos Montani
Turcato Junior, Gilberto [UNIFESP]
Accetturi, Conceicao Aparecida [UNIFESP]
Sabino, Ester Cerdeira [UNIFESP]
Instituto Adolfo Lutz
Universidade Federal de São Paulo (UNIFESP)
Fundação Pró-Sangue/Hemocentro de São Paulo
Centro de Referência de AIDS (CRAIDS)
Keywords: HIV-1 subtypes
intravenous drug users
molecular epidemiology
heteroduplex mobility analysis
Issue Date: 1-Jan-2001
Publisher: Associação Brasileira de Divulgação Científica
Citation: Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 1, p. 45-47, 2001.
Abstract: In order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative (6 samples from each group); low DNA concentration was the reason for non-amplification in half of these cases. Of the 42 typed cases from São Paulo, 34 (81%, 95% confidence limits 74.9 to 87.0%) were B and 8 (19%, 95% confidence limits 12.9 to 25.0%) were F, whereas of the 41 typed cases from Santos, 39 (95%, 95% confidence limits 91.6 to 98.4%) were B and 2 (5%, 95% confidence limits 1.6 to 8.4%) were C. We therefore confirm the relationship between clade F and intravenous drug use in São Paulo, and the presence of clade C in Santos. The fact that different genetic subtypes of HIV-1 are co-circulating indicates a need for continuous surveillance for these subtypes as well as for recombinant viruses in Brazil.
ISSN: 0100-879X
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