Random amplified polymorphic DNA profiles as a tool for the characterization of Brazilian keratitis isolates of the genus Acanthamoeba

Random amplified polymorphic DNA profiles as a tool for the characterization of Brazilian keratitis isolates of the genus Acanthamoeba

Autor Alves, J.m.p. Google Scholar
Gusmão, C.x. Google Scholar
Teixeira, M.m.g. Google Scholar
Freitas, Denise de Autor UNIFESP Google Scholar
Foronda, A.s. Google Scholar
Affonso, H.t. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo The genus Acanthamoeba comprises free-living amebae identified as opportunistic pathogens of humans and other animal species. Morphological, biochemical and molecular approaches have shown wide genetic diversity within the genus. In an attempt to determine the genetic relatedness among isolates of Acanthamoeba we analyzed randomly amplified polymorphic DNA (RAPD) profiles of 11 Brazilian isolates from cases of human keratitis and 8 American type culture collection (ATCC) reference strains. We found that ATCC strains belonging to the same species present polymorphic RAPD profiles whereas strains of different species show very similar profiles. Although most Brazilian isolates could not be assigned with certainty to any of the reference species, they could be clustered according to pattern similarities. The results show that RAPD analysis is a useful tool for the rapid characterization of new isolates and the assessment of genetic relatedness of Acanthamoeba spp. A comparison between RAPD analyses and morphological characteristics of cyst stages is also discussed.
Palavra-chave Acanthamoeba
keratitis
RAPD typing
genetic variation
Idioma Inglês
Data de publicação 2000-01-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 33, n. 1, p. 19-26, 2000.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 19-26
Fonte http://dx.doi.org/10.1590/S0100-879X2000000100003
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000085031800003
SciELO S0100-879X2000000100003 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/880

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